Matches in SemOpenAlex for { <https://semopenalex.org/work/W1481238272> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1481238272 endingPage "221" @default.
- W1481238272 startingPage "214" @default.
- W1481238272 abstract "1 A method is described for the preparation from Actinidia chinensis (Chinese gooseberry) fruit of a crystalline proteinase which catalyzes the hydrolysis of N2-benzoyl-l-arginine ethylester, N2-p-toluene-sulphonyl-l-glutamine p-nitrophenyl ester (used in the routine assay) and at least 15% of the peptide bonds of gelatin. The synthesis of the tosyl-glutamine p-nitrophenyl ester is described. The enzyme resembles papain in its action against benzoyl-l-arginine ethyl ester, having a broad pH-optimum from pH 5–7, with Km(app) of 89 mM and kcat of about 2.6 sec−1 at pH 5.6, 25° in 0.3 M KCl. 2 The enzymic activity elutes from Sephadex G-50 as a protein of molecular weight 12800 ± 700, and is partially resolved from inactive protein of apparent molecular wight 15400 ± 800. The preparation has minimum solubility near pH 3.1 and migrates at both pH 8.3 and 5.0 on polyacrylamide gel electrophoresis as two closely-spaced anionic bands. These two electrophoretic components are partially resolved by chromatography on DEAE-cellulose and both are enzymically active against tosyl-l-glutamine p-nitrophenyl ester. 3 The inhibitions of enzymic activity by 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) and 4,4′-dithiodipyridine, both reversible by 1,4-dithiothreitol, show that enzymic activity is dependent on an intact sulphhydryl group assaying about 0.23 moles per 12800 g protein. The enzyme is rapidly inactivated by iodoacetate and thereafter gives a low sulphhydryl assay with dithiodipyridine. Conversely, pretreatment with DTNB gives substantial protection against iodoacetate." @default.
- W1481238272 created "2016-06-24" @default.
- W1481238272 creator A5059313062 @default.
- W1481238272 date "1970-06-01" @default.
- W1481238272 modified "2023-10-07" @default.
- W1481238272 title "Anionic Proteinase from Actinidia chinensis. Preparation and Properties of the Crystalline Enzyme" @default.
- W1481238272 cites W112033475 @default.
- W1481238272 cites W1479681363 @default.
- W1481238272 cites W1480971655 @default.
- W1481238272 cites W153382533 @default.
- W1481238272 cites W1604618606 @default.
- W1481238272 cites W1873794413 @default.
- W1481238272 cites W1973181401 @default.
- W1481238272 cites W1980235078 @default.
- W1481238272 cites W1983421656 @default.
- W1481238272 cites W2000246688 @default.
- W1481238272 cites W2017321765 @default.
- W1481238272 cites W2030177071 @default.
- W1481238272 cites W2036474910 @default.
- W1481238272 cites W2058122435 @default.
- W1481238272 cites W2060284147 @default.
- W1481238272 cites W2062149436 @default.
- W1481238272 cites W2079438739 @default.
- W1481238272 cites W2083945806 @default.
- W1481238272 cites W2089964445 @default.
- W1481238272 cites W2092331199 @default.
- W1481238272 cites W2094363726 @default.
- W1481238272 cites W2314087427 @default.
- W1481238272 cites W2324725329 @default.
- W1481238272 cites W2410457102 @default.
- W1481238272 cites W2993022642 @default.
- W1481238272 cites W38976914 @default.
- W1481238272 doi "https://doi.org/10.1111/j.1432-1033.1970.tb00280.x" @default.
- W1481238272 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5506167" @default.
- W1481238272 hasPublicationYear "1970" @default.
- W1481238272 type Work @default.
- W1481238272 sameAs 1481238272 @default.
- W1481238272 citedByCount "100" @default.
- W1481238272 countsByYear W14812382722013 @default.
- W1481238272 countsByYear W14812382722014 @default.
- W1481238272 countsByYear W14812382722016 @default.
- W1481238272 countsByYear W14812382722017 @default.
- W1481238272 countsByYear W14812382722018 @default.
- W1481238272 countsByYear W14812382722021 @default.
- W1481238272 countsByYear W14812382722022 @default.
- W1481238272 crossrefType "journal-article" @default.
- W1481238272 hasAuthorship W1481238272A5059313062 @default.
- W1481238272 hasBestOaLocation W14812382721 @default.
- W1481238272 hasConcept C13965031 @default.
- W1481238272 hasConcept C178790620 @default.
- W1481238272 hasConcept C181199279 @default.
- W1481238272 hasConcept C185592680 @default.
- W1481238272 hasConcept C2777426643 @default.
- W1481238272 hasConcept C2777824588 @default.
- W1481238272 hasConcept C2779832904 @default.
- W1481238272 hasConcept C43617362 @default.
- W1481238272 hasConcept C538909803 @default.
- W1481238272 hasConcept C71240020 @default.
- W1481238272 hasConcept C94412978 @default.
- W1481238272 hasConceptScore W1481238272C13965031 @default.
- W1481238272 hasConceptScore W1481238272C178790620 @default.
- W1481238272 hasConceptScore W1481238272C181199279 @default.
- W1481238272 hasConceptScore W1481238272C185592680 @default.
- W1481238272 hasConceptScore W1481238272C2777426643 @default.
- W1481238272 hasConceptScore W1481238272C2777824588 @default.
- W1481238272 hasConceptScore W1481238272C2779832904 @default.
- W1481238272 hasConceptScore W1481238272C43617362 @default.
- W1481238272 hasConceptScore W1481238272C538909803 @default.
- W1481238272 hasConceptScore W1481238272C71240020 @default.
- W1481238272 hasConceptScore W1481238272C94412978 @default.
- W1481238272 hasIssue "2" @default.
- W1481238272 hasLocation W14812382721 @default.
- W1481238272 hasLocation W14812382722 @default.
- W1481238272 hasOpenAccess W1481238272 @default.
- W1481238272 hasPrimaryLocation W14812382721 @default.
- W1481238272 hasRelatedWork W1494023132 @default.
- W1481238272 hasRelatedWork W1976967260 @default.
- W1481238272 hasRelatedWork W2083715008 @default.
- W1481238272 hasRelatedWork W2350385321 @default.
- W1481238272 hasRelatedWork W2361207480 @default.
- W1481238272 hasRelatedWork W2362799233 @default.
- W1481238272 hasRelatedWork W2370522315 @default.
- W1481238272 hasRelatedWork W2380638270 @default.
- W1481238272 hasRelatedWork W2381798082 @default.
- W1481238272 hasRelatedWork W2384514363 @default.
- W1481238272 hasVolume "14" @default.
- W1481238272 isParatext "false" @default.
- W1481238272 isRetracted "false" @default.
- W1481238272 magId "1481238272" @default.
- W1481238272 workType "article" @default.