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- W1481339062 abstract "For establishment of simple and efficient method of virus elimination and production of virus-free bulblets, immature vegetative bulbils and their meristem-tip of involucre of garlic (Allium sativum L.) ‘Godang’ were cultured on LS medium supplemented with various concentration of plant growth regulators, polyamines and silver nitrate. Immature vegetative bulbils showed different growth patterns according to size and excision site in involucre. Immature vegetative bulbils in primary involucre formed direct bulblet formation without shoot proliferation, leaf elongation and root formation. However, immature vegetative bulbils in secondary and third involucre produced multiple shoot with leaf elongation and root formation. The best shoot proliferation and bulb formation of immature vegetative bulbils was shown in LS medium supplemented with 0.2 mgL-1 NAA and 5.0 mgL-1 2-iP. Addition of 5-10 μM silver nitrate in the media significantly accelerated shoot proliferation and bulb formation. Excision of meristem-tip from immature vegetative bulbils was simple and easy by elimination of one layer scale, compared with excision of dormant clove’s meristem. The best multiplication was achieved when meristem of immature vegetative bulbils cultured on LS medium supplemented with 0.2 mgL-1 NAA and 5.0 mgL-1 2-iP. On average, 6.8 shoots formed from one meristem. Examination of virus infection by reverse transcription polymerase chain reaction (RT-PCR) showed that viruses were not eliminated by immature vegetative bulbils culture, while viruses completely eliminated by meristem culture of immature vegetative bulbils." @default.
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- W1481339062 date "2006-01-01" @default.
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- W1481339062 title "Production of virus-free bulblets of garlic (Allium sativum L.) by meristem tip culture of immature vegetative bulbils." @default.
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