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• W1481505704 startingPage "3063" @default.
• W1481505704 abstract "The regulatory region of the Escherichia coli cya gene was analyzed by using S1 nuclease mapping and in vitro transcription experiments. The cya gene was transcribed, both in vivo and in vitro, from one major promoter (P2) and two weak promoters (P1 and P1') that are located about 200 base pairs upstream of P2. The transcription from P2 was specifically inhibited by cAMP-CRP (cAMP receptor protein) in vitro. This regulatory mechanism was shown to be physiologically relevant through quantitative analyses of the cya mRNA in intact cells by S1 and dot blot assays. DNase I protection experiments revealed that cAMP-CRP binds to the cya DNA region between +11 and -20, in which a consensus CRP binding sequence is present. Moreover, it was found that cAMP-CRP alters the binding of RNA polymerase to the promoter region, thus inhibiting the transcription of the cya gene." @default.
• W1481505704 created "2016-06-24" @default.
• W1481505704 creator A5030661869 @default.
• W1481505704 date "1985-03-01" @default.
• W1481505704 modified "2023-10-13" @default.
• W1481505704 title "Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein." @default.
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• W1481505704 doi "https://doi.org/10.1016/s0021-9258(18)89473-x" @default.
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