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- W1481815584 abstract "This chapter describes polymerase chain reaction (PCR) techniques to measure the proportion of mitochondrial DNA (mtDNA4977) to wild-type mtDNA. The quantitative method has been used to measure the levels of the deletion in different regions of the brains of aged individuals. The method can be used for the quantitation of other rare mtDNA deletions in brain or other tissues of humans or other species. The parameters for PCR need to be individually optimized and exponential ranges of amplification determined for each PCR target. Once these conditions are determined, the technique is quite reproducible. In diseases such as chronic progressive external ophthalmopelgia, Kearn-Sayre's syndrome, and Pearson's syndrome, a significant proportion (20-80%) of the mitochondrial DNA (mtDNA) carry large deletions of up to 10 kb. The chapter discusses PCR-based techniques used in the laboratory for the detection and semiquantitative and quantitative measurement of the levels of the mtDNA deletion, the so-called common deletion that removes 4977 bp of mtDNA sequence between two 13-bp repeats." @default.
- W1481815584 created "2016-06-24" @default.
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- W1481815584 date "1996-01-01" @default.
- W1481815584 modified "2023-10-17" @default.
- W1481815584 title "[36] Detection and quantification of mitochondrial DNA deletions" @default.
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- W1481815584 doi "https://doi.org/10.1016/s0076-6879(96)64038-5" @default.
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