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- W1482786539 abstract "The degree of activation of glass-adherent human blood monocyte-macrophages cultured with autologous lymphocytes was assessed by measurement of [14C]glucosamine uptake. In the absence of streptokinase-streptodornase (SK-SD) or purified protein derivative of tuberculin (PPD) minimal incorporation of the labeled compound occurred. Enhanced glucosamine uptake in the presence of antigen was positively correlated with cell donor-delayed skin hypersensitivity (PPD) and in vitro lymphoproliferative response (PPD, SK-SD). Increasing antigen or mononuclear cell concentrations resulted in increasing macrophage glucosamine uptake. Lymphoblasts, cell clumping, and macrophages with prominent pseudopodia were seen on stained monolayers of stimulated cells. Radioautography of such monolayers showed that the radiolabel was present only in mononuclear phagocytes. Adherent cell protein also generally increased in stimulated monolayers but did not account for the enhanced glucosamine uptake. Measurement of radioactive glucosamine incorporation into human macrophages is a useful tool to assess their degree of activation by lymphocytes stimulated by specific antigen." @default.
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- W1482786539 date "1980-07-01" @default.
- W1482786539 modified "2023-09-26" @default.
- W1482786539 title "A modified microassay for human macrophage activation" @default.
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- W1482786539 doi "https://doi.org/10.1016/0008-8749(80)90437-2" @default.
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