FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1482788894> ?p ?o ?g. }

• W1482788894 endingPage "12638" @default.
• W1482788894 startingPage "12633" @default.
• W1482788894 abstract "Prorenin, the inactive biosynthetic precursor of renin, is proteolytically cleaved in the renal juxtaglomerular cells to renin. The activity of renin is rate-limiting for generation of angiotensin II in the circulation. We identified a renal thiol protease which activates and accurately cleaves the 43-amino acid prosegment of human recombinant prorenin. In the current studies, 6.5 mg of this protease was purified from human renal cortex using a three-step procedure dependent upon Leu-Leu-arginyl affinity chromatography. This represented an overall 766-fold purification and resulted in three protein bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of molecular weights 30,000, 25,000, and 24,000. All three bands cross-reacted with an anti-human liver cathepsin B antibody upon immunoblot analysis; electrolution of each band and amino-terminal sequence analysis confirmed that the Mr 30,000 protein was mature cathepsin B and the Mr 25,000 and 24,000 bands were cathepsin B subunits. The pH optimum for the hydrolysis of pure human recombinant prorenin by pure renal cathepsin B was 6, and the Michaelis-Menten constant, Km, of the reaction was 1.4 x 10(-9) M. Immunostaining of human kidney using a sheep anti-human cathepsin B antibody demonstrated the presence of cathepsin B in the juxtaglomerular areas of the kidney, as well as in the renal proximal tubules. Electron microscopic immunohistochemistry using the same antibody demonstrated cathepsin B in dense secretory granules of the juxtaglomerular cells. Renin was also shown to be present in these granules. This study provides both biochemical and morphological evidence that renal cathepsin B is a human prorenin-processing enzyme." @default.
• W1482788894 created "2016-06-24" @default.
• W1482788894 creator A5004590878 @default.
• W1482788894 creator A5019496426 @default.
• W1482788894 creator A5020538559 @default.
• W1482788894 creator A5035986545 @default.
• W1482788894 creator A5037884146 @default.
• W1482788894 creator A5078500255 @default.
• W1482788894 creator A5083296600 @default.
• W1482788894 date "1991-07-01" @default.
• W1482788894 modified "2023-10-15" @default.
• W1482788894 title "Identification of renal cathepsin B as a human prorenin-processing enzyme" @default.
• W1482788894 cites W1484675846 @default.
• W1482788894 cites W1541743572 @default.
• W1482788894 cites W1586793828 @default.
• W1482788894 cites W1600871831 @default.
• W1482788894 cites W1750966629 @default.
• W1482788894 cites W192672120 @default.
• W1482788894 cites W1976644701 @default.
• W1482788894 cites W1983058218 @default.
• W1482788894 cites W1987985087 @default.
• W1482788894 cites W1991360681 @default.
• W1482788894 cites W2004381546 @default.
• W1482788894 cites W2027536811 @default.
• W1482788894 cites W2030391215 @default.
• W1482788894 cites W2044572816 @default.
• W1482788894 cites W2052406391 @default.
• W1482788894 cites W2059198543 @default.
• W1482788894 cites W2061144417 @default.
• W1482788894 cites W2063042519 @default.
• W1482788894 cites W2080142761 @default.
• W1482788894 cites W2087338783 @default.
• W1482788894 cites W2088191210 @default.
• W1482788894 cites W2094890678 @default.
• W1482788894 cites W2100837269 @default.
• W1482788894 cites W2103014408 @default.
• W1482788894 cites W2111396941 @default.
• W1482788894 cites W2111817213 @default.
• W1482788894 cites W2154944250 @default.
• W1482788894 cites W2424552696 @default.
• W1482788894 cites W2425821490 @default.
• W1482788894 cites W27304192 @default.
• W1482788894 cites W4293247451 @default.
• W1482788894 doi "https://doi.org/10.1016/s0021-9258(18)98946-5" @default.
• W1482788894 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2061332" @default.
• W1482788894 hasPublicationYear "1991" @default.
• W1482788894 type Work @default.
• W1482788894 sameAs 1482788894 @default.
• W1482788894 citedByCount "71" @default.
• W1482788894 countsByYear W14827888942012 @default.
• W1482788894 countsByYear W14827888942013 @default.
• W1482788894 countsByYear W14827888942014 @default.
• W1482788894 countsByYear W14827888942017 @default.
• W1482788894 countsByYear W14827888942018 @default.
• W1482788894 countsByYear W14827888942021 @default.
• W1482788894 countsByYear W14827888942022 @default.
• W1482788894 crossrefType "journal-article" @default.
• W1482788894 hasAuthorship W1482788894A5004590878 @default.
• W1482788894 hasAuthorship W1482788894A5019496426 @default.
• W1482788894 hasAuthorship W1482788894A5020538559 @default.
• W1482788894 hasAuthorship W1482788894A5035986545 @default.
• W1482788894 hasAuthorship W1482788894A5037884146 @default.
• W1482788894 hasAuthorship W1482788894A5078500255 @default.
• W1482788894 hasAuthorship W1482788894A5083296600 @default.
• W1482788894 hasBestOaLocation W14827888941 @default.
• W1482788894 hasConcept C116834253 @default.
• W1482788894 hasConcept C167844969 @default.
• W1482788894 hasConcept C173633252 @default.
• W1482788894 hasConcept C181199279 @default.
• W1482788894 hasConcept C185592680 @default.
• W1482788894 hasConcept C2780034444 @default.
• W1482788894 hasConcept C28021979 @default.
• W1482788894 hasConcept C51618164 @default.
• W1482788894 hasConcept C55493867 @default.
• W1482788894 hasConcept C59822182 @default.
• W1482788894 hasConcept C78553746 @default.
• W1482788894 hasConcept C86803240 @default.
• W1482788894 hasConceptScore W1482788894C116834253 @default.
• W1482788894 hasConceptScore W1482788894C167844969 @default.
• W1482788894 hasConceptScore W1482788894C173633252 @default.
• W1482788894 hasConceptScore W1482788894C181199279 @default.
• W1482788894 hasConceptScore W1482788894C185592680 @default.
• W1482788894 hasConceptScore W1482788894C2780034444 @default.
• W1482788894 hasConceptScore W1482788894C28021979 @default.
• W1482788894 hasConceptScore W1482788894C51618164 @default.
• W1482788894 hasConceptScore W1482788894C55493867 @default.
• W1482788894 hasConceptScore W1482788894C59822182 @default.
• W1482788894 hasConceptScore W1482788894C78553746 @default.
• W1482788894 hasConceptScore W1482788894C86803240 @default.
• W1482788894 hasIssue "19" @default.
• W1482788894 hasLocation W14827888941 @default.
• W1482788894 hasOpenAccess W1482788894 @default.
• W1482788894 hasPrimaryLocation W14827888941 @default.
• W1482788894 hasRelatedWork W1837500998 @default.
• W1482788894 hasRelatedWork W1996686686 @default.
• W1482788894 hasRelatedWork W2003121792 @default.
• W1482788894 hasRelatedWork W2013314062 @default.
• W1482788894 hasRelatedWork W2016317898 @default.

• next