Matches in SemOpenAlex for { <https://semopenalex.org/work/W1483009610> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1483009610 endingPage "4266" @default.
- W1483009610 startingPage "4258" @default.
- W1483009610 abstract "Abstract The solubilization and partial purification of β-galactosidase and β-glucuronidase from rabbit brain, using 4-methylumbelliferyl-β-d-galactopyranoside and 4-methylumbelliferyl-β-d-glucuronide as substrates, were achieved through preparation of an acetone powder, extraction of the powder with buffer, fractionation with ammonium sulfate, ion-exchange chromatography, and Sephadex gel filtration. It is shown that there are two different β-galactosidases and two different β-glucuronidases in rabbit brain active toward the mentioned substrates. The major β-galactosidase was purified approximately 500-fold over the homogenate and the β-glucuronidase approximately 3,000-fold. The purified enzymes were free of β-glucosidase activity. The most highly purified preparations of the two β-galactosidases also hydrolyzed o- and p-nitrophenyl-β-d-galactoside, and lactose but did not catalyze the hydrolysis of galactosylgalactosylglucosyl-ceramide and galactosylglucosyl-ceramide. The estimated molecular weights of the two galactosidases were 81,000 and 103,000, and of the β-glucuronidase, 266,000. A micromethod for the measurement of β-galactosidase activity when using complex glycosphingolipid substrates is described." @default.
- W1483009610 created "2016-06-24" @default.
- W1483009610 creator A5008096968 @default.
- W1483009610 creator A5016511745 @default.
- W1483009610 date "1968-08-01" @default.
- W1483009610 modified "2023-10-03" @default.
- W1483009610 title "Glycosidases in the Nervous System" @default.
- W1483009610 cites W1489283330 @default.
- W1483009610 cites W1535927809 @default.
- W1483009610 cites W1536351550 @default.
- W1483009610 cites W1551185066 @default.
- W1483009610 cites W1561641325 @default.
- W1483009610 cites W1590744179 @default.
- W1483009610 cites W1775749144 @default.
- W1483009610 cites W184703344 @default.
- W1483009610 cites W1940608734 @default.
- W1483009610 cites W1964818746 @default.
- W1483009610 cites W2003392649 @default.
- W1483009610 cites W2005282569 @default.
- W1483009610 cites W201503631 @default.
- W1483009610 cites W2026507072 @default.
- W1483009610 cites W2028978371 @default.
- W1483009610 cites W2030970393 @default.
- W1483009610 cites W2083587313 @default.
- W1483009610 cites W2087510544 @default.
- W1483009610 cites W2137302959 @default.
- W1483009610 cites W2152798248 @default.
- W1483009610 cites W2186669865 @default.
- W1483009610 cites W2292385629 @default.
- W1483009610 cites W2321069133 @default.
- W1483009610 cites W2338708608 @default.
- W1483009610 cites W2408181277 @default.
- W1483009610 cites W2420619318 @default.
- W1483009610 cites W2746686503 @default.
- W1483009610 cites W3020922752 @default.
- W1483009610 cites W4206035870 @default.
- W1483009610 cites W50363570 @default.
- W1483009610 doi "https://doi.org/10.1016/s0021-9258(18)93251-5" @default.
- W1483009610 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5679961" @default.
- W1483009610 hasPublicationYear "1968" @default.
- W1483009610 type Work @default.
- W1483009610 sameAs 1483009610 @default.
- W1483009610 citedByCount "63" @default.
- W1483009610 crossrefType "journal-article" @default.
- W1483009610 hasAuthorship W1483009610A5008096968 @default.
- W1483009610 hasAuthorship W1483009610A5016511745 @default.
- W1483009610 hasBestOaLocation W14830096101 @default.
- W1483009610 hasConcept C169760540 @default.
- W1483009610 hasConcept C185592680 @default.
- W1483009610 hasConcept C545706735 @default.
- W1483009610 hasConcept C55493867 @default.
- W1483009610 hasConcept C86803240 @default.
- W1483009610 hasConceptScore W1483009610C169760540 @default.
- W1483009610 hasConceptScore W1483009610C185592680 @default.
- W1483009610 hasConceptScore W1483009610C545706735 @default.
- W1483009610 hasConceptScore W1483009610C55493867 @default.
- W1483009610 hasConceptScore W1483009610C86803240 @default.
- W1483009610 hasIssue "16" @default.
- W1483009610 hasLocation W14830096101 @default.
- W1483009610 hasOpenAccess W1483009610 @default.
- W1483009610 hasPrimaryLocation W14830096101 @default.
- W1483009610 hasRelatedWork W1531601525 @default.
- W1483009610 hasRelatedWork W1990781990 @default.
- W1483009610 hasRelatedWork W2319480705 @default.
- W1483009610 hasRelatedWork W2384464875 @default.
- W1483009610 hasRelatedWork W2606230654 @default.
- W1483009610 hasRelatedWork W2607424097 @default.
- W1483009610 hasRelatedWork W2748952813 @default.
- W1483009610 hasRelatedWork W2899084033 @default.
- W1483009610 hasRelatedWork W2948807893 @default.
- W1483009610 hasRelatedWork W2778153218 @default.
- W1483009610 hasVolume "243" @default.
- W1483009610 isParatext "false" @default.
- W1483009610 isRetracted "false" @default.
- W1483009610 magId "1483009610" @default.
- W1483009610 workType "article" @default.