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- W1483115728 abstract "Oligodendrocytes are the myelinating cells of the central nervous system, and are avidly studied by investigators interested in neural cell lineages, myelination, multiple sclerosis, and oligodendroglioma. This unit presents a strategy for manipulating the division and differentiation of oligodendrocyte progenitors by isolating O-2A progenitors from optic nerve and then purifying and expanding them by culture in a defined medium supplemented with platelet-derived growth factor (PDGF) plus basic fibroblast growth factor (bFGF). Cells are then allowed to differentiate through removal of the growth factors. A protocol is also provided for monitoring the cell type composition of the cultures by immunohistochemistry. In addition to making it possible to generate large numbers of cells, this approach allows access to intermediate differentiation stages. Cells can thus be studied as they differentiate into oligodendrocytes, making the actual process of differentiation open to examination." @default.
- W1483115728 created "2016-06-24" @default.
- W1483115728 creator A5003934165 @default.
- W1483115728 date "1997-09-01" @default.
- W1483115728 modified "2023-10-16" @default.
- W1483115728 title "Isolation and Purification of Primary Oligodendrocyte Precursors" @default.
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- W1483115728 doi "https://doi.org/10.1002/0471142301.ns0304s00" @default.
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