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- W1483146423 abstract "Thermomonospora curvata contains α-1,4-glucosidase that is induced duringgrowth on maltose and starch. Maltose acts as an inducer of α-glucosidase even in thepresence of glucose. An intracellular thermostable α-glucosidase from T. curvata wasdetected in the crude extract on SDS-PAGE by means of modified colour reaction afterrenaturation of the enzyme. The enzyme was purified 59-fold to homogeneity with a yield of17·7% by a combination of ion-exchange and hydrophobic interaction chromatography andgel filtration. The enzyme has an apparent molecular mass of 60±1 kDa and isoelectric point4·1. The α-glucosidase exhibits optimum activity at pH 7·0–7·5 and54°C. The activity is inhibited by heavy metals and is positively affected by Ca2+ andMg2+. The enzyme hydrolyses maltose, sucrose, p-nitrophenyl-α-d-glucopyranoside and maltodextrins from maltotriose up to maltoheptaose with a decreasingefficiency. The Km for maltose and p-NPG are 12 and 2·3 mmol l−1,respectively." @default.
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- W1483146423 date "1997-09-01" @default.
- W1483146423 modified "2023-10-01" @default.
- W1483146423 title "Production and properties of ααα‐glucosidase from the thermotolerant bacterium <i>Thermomonospora curvata</i>" @default.
- W1483146423 doi "https://doi.org/10.1046/j.1365-2672.1997.00245.x" @default.
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