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• W1484191503 abstract "Abstract This unit describes how PCR can be used to exponentially amplify segments of DNA located between two specified primer hybridization sites. A single‐sided PCR method is used that initially requires specification of only one primer hybridization site; the second is defined by the ligation‐based addition of a unique DNA linker. This linker, together with the flanking gene‐specific primer, allows exponential amplification of any fragment of DNA. Because a defined, discrete‐length sequence is added to every fragment, complex populations of DNA such as sequence ladders can be amplified intact with retention of single‐base resolution. The ligation‐based protocol was specifically designed for genomic footprinting and direct sequencing reactions, and is described in this context; it can, however, be used for other applications." @default.
• W1484191503 created "2016-06-24" @default.
• W1484191503 creator A5017222943 @default.
• W1484191503 creator A5056820199 @default.
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• W1484191503 date "2001-10-01" @default.
• W1484191503 modified "2023-09-23" @default.
• W1484191503 title "Ligation‐Mediated <scp>PCR</scp> for Genomic Sequencing and Footprinting" @default.
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• W1484191503 doi "https://doi.org/10.1002/0471142727.mb1503s56" @default.
• W1484191503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18265117" @default.
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