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- W1484202205 abstract "Using adult rats, the characteristic transporting system for lactoferrin (LF) from intestinal lumen into the blood circulation was investigated. The rats were randomly divided into two groups, a non‐collected thoracic lymph (NC) group and a collected thoracic lymph (LC) group. Peripheral blood and thoracic lymph were collected from a jugular vein and a thoracic lymph duct, respectively, under anaesthesia. Bovine LF (bLF) was infused into the duodenal lumen by needle over a 1‐min period at a dose of 1 g kg −1 . The transported bLF in the plasma and lymph was assayed quantitatively by double‐antibody enzyme‐linked immunosorbent assay (ELISA). Morphological investigation was also carried out in the intestine, lymph node, and liver. Following intraduodenal administration of bLF, the transported bLF in the NC group was detected in the plasma, and reached a peak value at 2 h. Furthermore, the bLF concentration in the thoracic duct lymph fluid in the LC group increased significantly, and peaked 2 h after the administration. In addition, bLF was not detected in the plasma of the LC group. Immunohistochemical analysis clearly showed anti‐bLF positive particles in the epithelial cells of the apical villi. The striated border and baso‐lateral membrane were also bLF positive. These results suggest that intraduodenally infused bLF is transported into the blood circulation via the lymphatic pathway, not via portal circulation in adult rats." @default.
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- W1484202205 date "2004-04-21" @default.
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- W1484202205 title "Evidence of lactoferrin transportation into blood circulation from intestine via lymphatic pathway in adult rats" @default.
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- W1484202205 doi "https://doi.org/10.1113/expphysiol.2003.026633" @default.
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