FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1484953871> ?p ?o ?g. }

• W1484953871 endingPage "R165" @default.
• W1484953871 startingPage "R165" @default.
• W1484953871 abstract "The healthy synovial lining layer consists of a single cell layer that regulates the transport between the joint cavity and the surrounding tissue. It has been suggested that abnormalities such as somatic mutations in the p53 tumor-suppressor gene contribute to synovial hyperplasia and invasion in rheumatoid arthritis (RA). In this study, expression of epithelial markers on healthy and diseased synovial lining tissue was examined. In addition, we investigated whether a regulated process, resembling epithelial to mesenchymal transition (EMT)/fibrosis, could be responsible for the altered phenotype of the synovial lining layer in RA. Synovial tissue from healthy subjects and RA patients was obtained during arthroscopy. To detect signs of EMT, expression of E-cadherin (epithelial marker), collagen type IV (indicator of the presence of a basement membrane) and α-smooth muscle actin (α-sma; a myofibroblast marker) was investigated on frozen tissue sections using immunohistochemistry. Fibroblast-like synoviocytes (FLSs) from healthy subjects were isolated and subjected to stimulation with synovial fluid (SF) from two RA patients and to transforming growth factor (TGF)-β. To detect whether EMT/fibrotic markers were increased, expression of collagen type I, α-sma and telopeptide lysylhydroxylase (TLH) was measured by real time PCR. Expression of E-cadherin and collagen type IV was found in healthy and arthritic synovial tissue. Expression of α-sma was only found in the synovial lining layer of RA patients. Stimulation of healthy FLSs with SF resulted in an upregulation of α-sma and TLH mRNA. Collagen type I and TLH mRNA were upregulated after stimulation with TGF-β. Addition of bone morphogenetic protein (BMP)-7 to healthy FLS stimulated with SF inhibited the expression of α-sma mRNA. The finding that E-cadherin and collagen type IV are expressed in the lining layer of healthy and arthritic synovium indicates that these lining cells display an epithelial-like phenotype. In addition, the presence of α-sma in the synovial lining layer of RA patients and induction of fibrotic markers in healthy FLSs by SF from RA patients indicate that a regulated process comparable to EMT might cause the alteration in phenotype of RA FLSs. Therefore, BMP-7 may represent a promising agent to counteract the transition imposed on synoviocytes in the RA joint." @default.
• W1484953871 created "2016-06-24" @default.
• W1484953871 creator A5000644268 @default.
• W1484953871 creator A5016120320 @default.
• W1484953871 creator A5022177606 @default.
• W1484953871 creator A5031449804 @default.
• W1484953871 creator A5049437946 @default.
• W1484953871 creator A5052842332 @default.
• W1484953871 creator A5072311778 @default.
• W1484953871 creator A5081825299 @default.
• W1484953871 creator A5083709043 @default.
• W1484953871 creator A5085468214 @default.
• W1484953871 creator A5090475741 @default.
• W1484953871 date "2006-01-01" @default.
• W1484953871 modified "2023-10-13" @default.
• W1484953871 cites W138924828 @default.
• W1484953871 cites W1493098149 @default.
• W1484953871 cites W1535381420 @default.
• W1484953871 cites W1786515414 @default.
• W1484953871 cites W1970037245 @default.
• W1484953871 cites W1987467902 @default.
• W1484953871 cites W1987740056 @default.
• W1484953871 cites W1990336673 @default.
• W1484953871 cites W1993345123 @default.
• W1484953871 cites W2007901974 @default.
• W1484953871 cites W2013169986 @default.
• W1484953871 cites W2020271584 @default.
• W1484953871 cites W2021597465 @default.
• W1484953871 cites W2025820825 @default.
• W1484953871 cites W2026158123 @default.
• W1484953871 cites W2032124801 @default.
• W1484953871 cites W2032942532 @default.
• W1484953871 cites W2033912622 @default.
• W1484953871 cites W2046401692 @default.
• W1484953871 cites W2051696368 @default.
• W1484953871 cites W2057325749 @default.
• W1484953871 cites W2065427271 @default.
• W1484953871 cites W2076869157 @default.
• W1484953871 cites W2086350454 @default.
• W1484953871 cites W2088876544 @default.
• W1484953871 cites W2090096683 @default.
• W1484953871 cites W2090489482 @default.
• W1484953871 cites W2091517245 @default.
• W1484953871 cites W2093714926 @default.
• W1484953871 cites W2102319255 @default.
• W1484953871 cites W2103224375 @default.
• W1484953871 cites W2108360007 @default.
• W1484953871 cites W2116576448 @default.
• W1484953871 cites W2120374931 @default.
• W1484953871 cites W2123233663 @default.
• W1484953871 cites W2128705963 @default.
• W1484953871 cites W2135418150 @default.
• W1484953871 cites W2143183772 @default.
• W1484953871 cites W2146726643 @default.
• W1484953871 cites W2147016321 @default.
• W1484953871 cites W2152635557 @default.
• W1484953871 cites W2158130175 @default.
• W1484953871 cites W2184817776 @default.
• W1484953871 cites W2196454906 @default.
• W1484953871 cites W2417367631 @default.
• W1484953871 cites W2465198679 @default.
• W1484953871 cites W2597632520 @default.
• W1484953871 doi "https://doi.org/10.1186/ar2073" @default.
• W1484953871 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1794508" @default.
• W1484953871 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17076892" @default.
• W1484953871 hasPublicationYear "2006" @default.
• W1484953871 type Work @default.
• W1484953871 sameAs 1484953871 @default.
• W1484953871 citedByCount "82" @default.
• W1484953871 countsByYear W14849538712012 @default.
• W1484953871 countsByYear W14849538712013 @default.
• W1484953871 countsByYear W14849538712014 @default.
• W1484953871 countsByYear W14849538712015 @default.
• W1484953871 countsByYear W14849538712016 @default.
• W1484953871 countsByYear W14849538712017 @default.
• W1484953871 countsByYear W14849538712018 @default.
• W1484953871 countsByYear W14849538712019 @default.
• W1484953871 countsByYear W14849538712020 @default.
• W1484953871 countsByYear W14849538712021 @default.
• W1484953871 countsByYear W14849538712022 @default.
• W1484953871 countsByYear W14849538712023 @default.
• W1484953871 crossrefType "journal-article" @default.
• W1484953871 hasAuthorship W1484953871A5000644268 @default.
• W1484953871 hasAuthorship W1484953871A5016120320 @default.
• W1484953871 hasAuthorship W1484953871A5022177606 @default.
• W1484953871 hasAuthorship W1484953871A5031449804 @default.
• W1484953871 hasAuthorship W1484953871A5049437946 @default.
• W1484953871 hasAuthorship W1484953871A5052842332 @default.
• W1484953871 hasAuthorship W1484953871A5072311778 @default.
• W1484953871 hasAuthorship W1484953871A5081825299 @default.
• W1484953871 hasAuthorship W1484953871A5083709043 @default.
• W1484953871 hasAuthorship W1484953871A5085468214 @default.
• W1484953871 hasAuthorship W1484953871A5090475741 @default.
• W1484953871 hasBestOaLocation W14849538711 @default.
• W1484953871 hasConcept C104317684 @default.
• W1484953871 hasConcept C126322002 @default.
• W1484953871 hasConcept C127561419 @default.
• W1484953871 hasConcept C142724271 @default.

• next