FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1485079524> ?p ?o ?g. }

• W1485079524 endingPage "7194" @default.
• W1485079524 startingPage "7188" @default.
• W1485079524 abstract "The role of protein kinase C in activation of the plasma membrane Na+/H+ exchanger was studied in cultured vascular smooth muscle cells. The basic lipid, sphingosine, was used to block enzymatic activity of protein kinase C. Na+/H+ exchange was activated by phorbol 12-myristate 13-acetate (PMA), diacylglycerols, platelet-derived growth factor (PDGF), thrombin, or by osmotically-induced cell shrinkage. Intracellular pH and Na+/H+ exchange activity were measured using the intracellular pH indicator, 2',7'-bis(carboxyethyl)-5(6) carboxyfluorescein. Acting alone, both crude sphingosine and pure, synthetic C18 D-(+)-erythro-sphingosine raised pHi in a dose-dependent manner (from 6.95 +/- 0.02 to 7.19 +/- 0.09 over 10 min for 10 microM sphingosine). This alkalinization was not due to Na+/H+ exchange as it was not altered by t-butylamiloride (50 microM) nor by replacement of the assay medium with a Na(+)-free solution. Sphingosine-induced alkalinization did not require protein kinase C activity, since it was fully intact in protein kinase C-depleted cells. It was also not due to a detergent action of sphingosine on the cell membrane, since both ionic and non-ionic detergents caused cell acidification. Rather, alkalinization induced by sphingosine appeared to be due to cellular uptake of NH3 groups since N-acetylsphingosine showed no alkalinization. After the initial cell alkalinization, cellular uptake of [3H]sphingosine continued slowly for up to 24 h. The ability of PMA or dioctanoylglycerol to activate Na+/H+ exchange fell to 20% of control after 24 h of sphingosine exposure. At all times, C11 and N-acetylsphingosine failed to block PMA-induced activation of the exchanger. Activation of the Na+/H+ exchanger by sucrose, which does not depend on protein kinase C activity, was unaffected by sphingosine. Activation of Na+/H+ exchange by thrombin and PDGF was partially inhibited by 30 and 20%, respectively. These data indicate that both thrombin and PDGF activate Na+/H+ exchange by pathway(s) that are primarily independent of protein kinase C." @default.
• W1485079524 created "2016-06-24" @default.
• W1485079524 creator A5033561618 @default.
• W1485079524 creator A5080225643 @default.
• W1485079524 creator A5082292595 @default.
• W1485079524 date "1990-05-01" @default.
• W1485079524 modified "2023-09-27" @default.
• W1485079524 title "Sphingosine differentially inhibits activation of the Na+/H+ exchanger by phorbol esters and growth factors." @default.
• W1485079524 cites W1493516959 @default.
• W1485079524 cites W1524495054 @default.
• W1485079524 cites W1534398269 @default.
• W1485079524 cites W1555606052 @default.
• W1485079524 cites W1595133660 @default.
• W1485079524 cites W1595529083 @default.
• W1485079524 cites W1604268538 @default.
• W1485079524 cites W1663970427 @default.
• W1485079524 cites W1970441944 @default.
• W1485079524 cites W1971429384 @default.
• W1485079524 cites W1974877407 @default.
• W1485079524 cites W1977038914 @default.
• W1485079524 cites W1990681528 @default.
• W1485079524 cites W2001692359 @default.
• W1485079524 cites W2003166129 @default.
• W1485079524 cites W2012003377 @default.
• W1485079524 cites W2013199351 @default.
• W1485079524 cites W2013430170 @default.
• W1485079524 cites W2014477576 @default.
• W1485079524 cites W2017223527 @default.
• W1485079524 cites W2023037230 @default.
• W1485079524 cites W2023978323 @default.
• W1485079524 cites W2024604911 @default.
• W1485079524 cites W2030975022 @default.
• W1485079524 cites W2038917226 @default.
• W1485079524 cites W2055892080 @default.
• W1485079524 cites W2057595921 @default.
• W1485079524 cites W2061574494 @default.
• W1485079524 cites W2068570002 @default.
• W1485079524 cites W2105369923 @default.
• W1485079524 cites W2135241184 @default.
• W1485079524 cites W2155447439 @default.
• W1485079524 cites W4238605098 @default.
• W1485079524 doi "https://doi.org/10.1016/s0021-9258(19)39097-0" @default.
• W1485079524 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2158991" @default.
• W1485079524 hasPublicationYear "1990" @default.
• W1485079524 type Work @default.
• W1485079524 sameAs 1485079524 @default.
• W1485079524 citedByCount "38" @default.
• W1485079524 countsByYear W14850795242012 @default.
• W1485079524 countsByYear W14850795242013 @default.
• W1485079524 crossrefType "journal-article" @default.
• W1485079524 hasAuthorship W1485079524A5033561618 @default.
• W1485079524 hasAuthorship W1485079524A5080225643 @default.
• W1485079524 hasAuthorship W1485079524A5082292595 @default.
• W1485079524 hasBestOaLocation W14850795241 @default.
• W1485079524 hasConcept C170493617 @default.
• W1485079524 hasConcept C185592680 @default.
• W1485079524 hasConcept C195794163 @default.
• W1485079524 hasConcept C2778703144 @default.
• W1485079524 hasConcept C2780043322 @default.
• W1485079524 hasConcept C3017695315 @default.
• W1485079524 hasConcept C55493867 @default.
• W1485079524 hasConcept C62478195 @default.
• W1485079524 hasConcept C86803240 @default.
• W1485079524 hasConcept C95444343 @default.
• W1485079524 hasConceptScore W1485079524C170493617 @default.
• W1485079524 hasConceptScore W1485079524C185592680 @default.
• W1485079524 hasConceptScore W1485079524C195794163 @default.
• W1485079524 hasConceptScore W1485079524C2778703144 @default.
• W1485079524 hasConceptScore W1485079524C2780043322 @default.
• W1485079524 hasConceptScore W1485079524C3017695315 @default.
• W1485079524 hasConceptScore W1485079524C55493867 @default.
• W1485079524 hasConceptScore W1485079524C62478195 @default.
• W1485079524 hasConceptScore W1485079524C86803240 @default.
• W1485079524 hasConceptScore W1485079524C95444343 @default.
• W1485079524 hasIssue "13" @default.
• W1485079524 hasLocation W14850795241 @default.
• W1485079524 hasOpenAccess W1485079524 @default.
• W1485079524 hasPrimaryLocation W14850795241 @default.
• W1485079524 hasRelatedWork W1514664656 @default.
• W1485079524 hasRelatedWork W1852213635 @default.
• W1485079524 hasRelatedWork W1965965826 @default.
• W1485079524 hasRelatedWork W1993608139 @default.
• W1485079524 hasRelatedWork W1996033915 @default.
• W1485079524 hasRelatedWork W2040317797 @default.
• W1485079524 hasRelatedWork W2074613314 @default.
• W1485079524 hasRelatedWork W2286885533 @default.
• W1485079524 hasRelatedWork W2406150497 @default.
• W1485079524 hasRelatedWork W4255836249 @default.
• W1485079524 hasVolume "265" @default.
• W1485079524 isParatext "false" @default.
• W1485079524 isRetracted "false" @default.
• W1485079524 magId "1485079524" @default.
• W1485079524 workType "article" @default.