FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1485299849> ?p ?o ?g. }

• W1485299849 endingPage "2537" @default.
• W1485299849 startingPage "2528" @default.
• W1485299849 abstract "Abstract Mechanical strain provides an anti-adipogenic, pro-osteogenic stimulus to mesenchymal stem cells (MSC) through generating intracellular signals and via cytoskeletal restructuring. Recently, mTORC2 has been shown to be a novel mechanical target critical for the anti-adipogenic signal leading to preservation of β-catenin. As mechanical activation of mTORC2 requires focal adhesions (FAs), we asked whether proximal signaling involved Src and FAK, which are early responders to integrin-FA engagement. Application of mechanical strain to marrow-derived MSCs was unable to activate mTORC2 when Src family kinases were inhibited. Fyn, but not Src, was specifically required for mechanical activation of mTORC2 and was recruited to FAs after strain. Activation of mTORC2 was further diminished following FAK inhibition, and as FAK phosphorylation (Tyr-397) required Fyn activity, provided evidence of Fyn/FAK cooperativity. Inhibition of Fyn also prevented mechanical activation of RhoA as well as mechanically induced actin stress fiber formation. We thus asked whether RhoA activation by strain was dependent on mTORC2 downstream of Fyn. Inhibition of mTORC2 or its downstream substrate, Akt, both prevented mechanical RhoA activation, indicating that Fyn/FAK affects cytoskeletal structure via mTORC2. We then sought to ascertain whether this Fyn-initiated signal pathway modulated MSC lineage decisions. siRNA knockdown of Fyn, but not Src, led to rapid attainment of adipogenic phenotype with significant increases in adipocyte protein 2, peroxisome proliferator-activated receptor gamma, adiponectin, and perilipin. As such, Fyn expression in mdMSCs contributes to basal cytoskeletal architecture and, when associated with FAs, functions as a proximal mechanical effector for environmental signals that influence MSC lineage allocation." @default.
• W1485299849 created "2016-06-24" @default.
• W1485299849 creator A5003554743 @default.
• W1485299849 creator A5009662300 @default.
• W1485299849 creator A5012529101 @default.
• W1485299849 creator A5015356773 @default.
• W1485299849 creator A5023092411 @default.
• W1485299849 creator A5026488724 @default.
• W1485299849 creator A5026948526 @default.
• W1485299849 creator A5027229690 @default.
• W1485299849 creator A5065838160 @default.
• W1485299849 creator A5070581832 @default.
• W1485299849 creator A5070642099 @default.
• W1485299849 date "2013-11-01" @default.
• W1485299849 modified "2023-10-02" @default.
• W1485299849 title "Mechanically activated fyn utilizes mTORC2 to regulate RhoA and adipogenesis in mesenchymal stem cells" @default.
• W1485299849 cites W1900470435 @default.
• W1485299849 cites W1965840684 @default.
• W1485299849 cites W1970990473 @default.
• W1485299849 cites W1977764445 @default.
• W1485299849 cites W1979615123 @default.
• W1485299849 cites W1982849870 @default.
• W1485299849 cites W1984721835 @default.
• W1485299849 cites W1985617800 @default.
• W1485299849 cites W2003035047 @default.
• W1485299849 cites W2007719816 @default.
• W1485299849 cites W2009843711 @default.
• W1485299849 cites W2010148876 @default.
• W1485299849 cites W2016872125 @default.
• W1485299849 cites W2021903494 @default.
• W1485299849 cites W2026516383 @default.
• W1485299849 cites W2042020095 @default.
• W1485299849 cites W2043224349 @default.
• W1485299849 cites W2045795713 @default.
• W1485299849 cites W2049279973 @default.
• W1485299849 cites W2051729750 @default.
• W1485299849 cites W2054779757 @default.
• W1485299849 cites W2057415995 @default.
• W1485299849 cites W2065887259 @default.
• W1485299849 cites W2070439333 @default.
• W1485299849 cites W2100563107 @default.
• W1485299849 cites W2107988120 @default.
• W1485299849 cites W2124169597 @default.
• W1485299849 cites W2126767664 @default.
• W1485299849 cites W2126838501 @default.
• W1485299849 cites W2134208433 @default.
• W1485299849 cites W2134807132 @default.
• W1485299849 cites W2135096442 @default.
• W1485299849 cites W2139855443 @default.
• W1485299849 cites W2144023435 @default.
• W1485299849 cites W2150102283 @default.
• W1485299849 cites W2150403223 @default.
• W1485299849 cites W2151802152 @default.
• W1485299849 cites W2158179525 @default.
• W1485299849 cites W2158196548 @default.
• W1485299849 cites W2165673443 @default.
• W1485299849 cites W2171997500 @default.
• W1485299849 cites W4238567307 @default.
• W1485299849 doi "https://doi.org/10.1002/stem.1476" @default.
• W1485299849 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4040149" @default.
• W1485299849 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23836527" @default.
• W1485299849 hasPublicationYear "2013" @default.
• W1485299849 type Work @default.
• W1485299849 sameAs 1485299849 @default.
• W1485299849 citedByCount "60" @default.
• W1485299849 countsByYear W14852998492014 @default.
• W1485299849 countsByYear W14852998492015 @default.
• W1485299849 countsByYear W14852998492016 @default.
• W1485299849 countsByYear W14852998492017 @default.
• W1485299849 countsByYear W14852998492018 @default.
• W1485299849 countsByYear W14852998492019 @default.
• W1485299849 countsByYear W14852998492020 @default.
• W1485299849 countsByYear W14852998492021 @default.
• W1485299849 countsByYear W14852998492022 @default.
• W1485299849 countsByYear W14852998492023 @default.
• W1485299849 crossrefType "journal-article" @default.
• W1485299849 hasAuthorship W1485299849A5003554743 @default.
• W1485299849 hasAuthorship W1485299849A5009662300 @default.
• W1485299849 hasAuthorship W1485299849A5012529101 @default.
• W1485299849 hasAuthorship W1485299849A5015356773 @default.
• W1485299849 hasAuthorship W1485299849A5023092411 @default.
• W1485299849 hasAuthorship W1485299849A5026488724 @default.
• W1485299849 hasAuthorship W1485299849A5026948526 @default.
• W1485299849 hasAuthorship W1485299849A5027229690 @default.
• W1485299849 hasAuthorship W1485299849A5065838160 @default.
• W1485299849 hasAuthorship W1485299849A5070581832 @default.
• W1485299849 hasAuthorship W1485299849A5070642099 @default.
• W1485299849 hasBestOaLocation W14852998492 @default.
• W1485299849 hasConcept C107846503 @default.
• W1485299849 hasConcept C108636557 @default.
• W1485299849 hasConcept C142669718 @default.
• W1485299849 hasConcept C1491633281 @default.
• W1485299849 hasConcept C2777093181 @default.
• W1485299849 hasConcept C2993400109 @default.
• W1485299849 hasConcept C37686147 @default.
• W1485299849 hasConcept C55493867 @default.
• W1485299849 hasConcept C62478195 @default.
• W1485299849 hasConcept C86554907 @default.

• next