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• W1485367674 abstract "Summary The aim of this investigation was to explore the pH‐induced conformational changes of phaseolin using differential scanning calorimetry (DSC), fluorescence and circular dichroism (CD) spectra. The surface charges at the varying pH and ionic strength were also determined to probe the possible relationship between the surface charges of phaseolin and its conformation. The ζ‐potential of phaseolin decreased from 27.9 mV at pH 3.0 to –44 mV at pH 9.4, and the isoelectric point (iep) occurred at pH 4.21, which were comparable with the theoretical estimations derived from amino acid composition and p K values. The iep of phaseolin was independent of ionic strength, while the magnitude of ζ‐potential decreased as the ionic strength increased. The near‐UV CD, DSC and fluorescence spectra analyses indicated highly acidic and alkaline pH conditions induced the loss of tertiary conformation of phaseolin. The far‐UV CD spectra analysis confirmed pH‐shift led to the decreases in ordered secondary structure compositions. The present results suggested the loss of secondary and/or tertiary conformation of phaseolin as a result of the varying pattern in electrostatic interactions as pH shifted from 7.0 to acidic or alkalic pH." @default.
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• W1485367674 date "2011-05-20" @default.
• W1485367674 modified "2023-10-17" @default.
• W1485367674 title "Surface charge and conformational properties of phaseolin, the major globulin in red kidney bean (Phaseolus vulgaris L): effect of pH" @default.
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• W1485367674 doi "https://doi.org/10.1111/j.1365-2621.2011.02662.x" @default.
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