Matches in SemOpenAlex for { <https://semopenalex.org/work/W1485681622> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1485681622 abstract "The effect of various galactose derivatives, substituted at C-6, on the phosphoenolpyruvate:beta-galactoside phosphotransferase system of Staphylococcus aureus was studied. Cells were grown by an improved procedure, which resulted in a 5- to 10-fold increase in cell yield. The four protein components of the system were separated. A membrane fraction containing negligible levels of the soluble components was prepared by alternate cycles of sonic treatment and differential centrifugation. The in vitro system reconstituted from these fractions was used to test the ability of the galactose derivatives to inhibit the phosphorylation of lactose analogs, under conditions where the membrane-bound component, Enzyme IIlac, was rate limiting. Derivaites in which the hydroxyl group of C-6 was missing, or replaced by a fluoro, O-methyl, or carboxyl group had no affinity for Enzyme IIlac, as judged by their inability to inhibit phosphorylation. Surprisingly, derivatives containing arylsulfonyl groups at C-6 were potent inhibitors; the O-tosyl compound has an apparent affinity five times that of galactose. The arylsulfonyl substitution in an absolute requirement; neither O-benzyl or O-methanesulfonyl derivatives were inhibitory. The specificity of the inhibition by tosyl derivatives parallels that of unsubstituted substrates; tosyl galactosides of the beta configuration were inhibitory, but those of the alpha configuration were not. The tosyl derivatives also strongly inhibited the uptake of lactose analogs into whole cells; the requirement for the arylsulfonyl moiety was again observed. The chemical analogy between the tosyl galactosides and possible intermediates in the transport-phosphorylation step catalyzed by Enzyme IIlac provides a possible explanation for the unexpected properties of these derivatives." @default.
- W1485681622 created "2016-06-24" @default.
- W1485681622 creator A5029487795 @default.
- W1485681622 creator A5062413243 @default.
- W1485681622 creator A5077886650 @default.
- W1485681622 date "1975-11-01" @default.
- W1485681622 modified "2023-09-28" @default.
- W1485681622 title "Inhibition by 6-O-tosyl galactosides of beta-galactoside phosphorylation and transport by the lactose phosphotransferase system of Staphylococcus aureus." @default.
- W1485681622 cites W1490306766 @default.
- W1485681622 cites W1506043065 @default.
- W1485681622 cites W1515591027 @default.
- W1485681622 cites W1568190283 @default.
- W1485681622 cites W1573772031 @default.
- W1485681622 cites W1580422367 @default.
- W1485681622 cites W1585454356 @default.
- W1485681622 cites W1592242797 @default.
- W1485681622 cites W1637283425 @default.
- W1485681622 cites W1975186487 @default.
- W1485681622 cites W1986257664 @default.
- W1485681622 cites W1991288560 @default.
- W1485681622 cites W1993909284 @default.
- W1485681622 cites W2000781125 @default.
- W1485681622 cites W2014975886 @default.
- W1485681622 cites W2040786978 @default.
- W1485681622 cites W2047159593 @default.
- W1485681622 cites W2054537516 @default.
- W1485681622 cites W2060321428 @default.
- W1485681622 cites W2069917813 @default.
- W1485681622 cites W2090722062 @default.
- W1485681622 cites W2094604572 @default.
- W1485681622 doi "https://doi.org/10.1016/s0021-9258(19)40749-7" @default.
- W1485681622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1184591" @default.
- W1485681622 hasPublicationYear "1975" @default.
- W1485681622 type Work @default.
- W1485681622 sameAs 1485681622 @default.
- W1485681622 citedByCount "14" @default.
- W1485681622 countsByYear W14856816222014 @default.
- W1485681622 countsByYear W14856816222022 @default.
- W1485681622 countsByYear W14856816222023 @default.
- W1485681622 crossrefType "journal-article" @default.
- W1485681622 hasAuthorship W1485681622A5029487795 @default.
- W1485681622 hasAuthorship W1485681622A5062413243 @default.
- W1485681622 hasAuthorship W1485681622A5077886650 @default.
- W1485681622 hasBestOaLocation W14856816221 @default.
- W1485681622 hasConcept C143937172 @default.
- W1485681622 hasConcept C181199279 @default.
- W1485681622 hasConcept C185592680 @default.
- W1485681622 hasConcept C2776392439 @default.
- W1485681622 hasConcept C2776568683 @default.
- W1485681622 hasConcept C2778173381 @default.
- W1485681622 hasConcept C2778718427 @default.
- W1485681622 hasConcept C2778724459 @default.
- W1485681622 hasConcept C2910581434 @default.
- W1485681622 hasConcept C45656491 @default.
- W1485681622 hasConcept C55493867 @default.
- W1485681622 hasConcept C71240020 @default.
- W1485681622 hasConceptScore W1485681622C143937172 @default.
- W1485681622 hasConceptScore W1485681622C181199279 @default.
- W1485681622 hasConceptScore W1485681622C185592680 @default.
- W1485681622 hasConceptScore W1485681622C2776392439 @default.
- W1485681622 hasConceptScore W1485681622C2776568683 @default.
- W1485681622 hasConceptScore W1485681622C2778173381 @default.
- W1485681622 hasConceptScore W1485681622C2778718427 @default.
- W1485681622 hasConceptScore W1485681622C2778724459 @default.
- W1485681622 hasConceptScore W1485681622C2910581434 @default.
- W1485681622 hasConceptScore W1485681622C45656491 @default.
- W1485681622 hasConceptScore W1485681622C55493867 @default.
- W1485681622 hasConceptScore W1485681622C71240020 @default.
- W1485681622 hasLocation W14856816221 @default.
- W1485681622 hasOpenAccess W1485681622 @default.
- W1485681622 hasPrimaryLocation W14856816221 @default.
- W1485681622 hasRelatedWork W1483346380 @default.
- W1485681622 hasRelatedWork W1515591027 @default.
- W1485681622 hasRelatedWork W1518598430 @default.
- W1485681622 hasRelatedWork W1568190283 @default.
- W1485681622 hasRelatedWork W1573772031 @default.
- W1485681622 hasRelatedWork W1580422367 @default.
- W1485681622 hasRelatedWork W1585454356 @default.
- W1485681622 hasRelatedWork W1623926801 @default.
- W1485681622 hasRelatedWork W1637283425 @default.
- W1485681622 hasRelatedWork W1979506814 @default.
- W1485681622 hasRelatedWork W1991288560 @default.
- W1485681622 hasRelatedWork W1995581506 @default.
- W1485681622 hasRelatedWork W2009203580 @default.
- W1485681622 hasRelatedWork W2034123321 @default.
- W1485681622 hasRelatedWork W2053084573 @default.
- W1485681622 hasRelatedWork W2060116740 @default.
- W1485681622 hasRelatedWork W2068281933 @default.
- W1485681622 hasRelatedWork W2150453547 @default.
- W1485681622 hasRelatedWork W2333288851 @default.
- W1485681622 hasRelatedWork W2891562629 @default.
- W1485681622 isParatext "false" @default.
- W1485681622 isRetracted "false" @default.
- W1485681622 magId "1485681622" @default.
- W1485681622 workType "article" @default.