FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1485697910> ?p ?o ?g. }

• W1485697910 endingPage "8774" @default.
• W1485697910 startingPage "8766" @default.
• W1485697910 abstract "The composition of the two-chain coiled-coil molecule of murine epidermal keratin intermediate filaments (KIF) containing keratins 1 (type II) and 10 (type I) has been explored using native-type KIF as well as KIF reassembled in vitro from protein dissolved in urea solutions or from mixtures of 3H-labeled and unlabeled purified chains. By use of cross-linking, high resolution polyacrylamide gel electrophoresis and blotting for 3H-labeled keratins or with an anti-mouse keratin 10 antibody, it was found that individual keratin chains form type I or type II homodimers and homotetramers in solution that do not assemble into KIF in vitro. When mixed in urea solutions of 5 M or greater, such homo-oligomers rapidly rearrange into mostly heterodimers and heterotetramers that support filament assembly. On the other hand, prekeratin, isolated from newborn mouse epidermis with 0.1 M sodium citrate buffer, pH 2.6, under conditions that do not dissociate the native coiled-coil molecule, consists exclusively of type I-II heterodimers and heterotetramers. It is necessary to dissolve prekeratin in 8-9.5 M urea for several hours in order to dissociate the native heterodimer molecule and incorporate tracer amounts of a single 3H-labeled keratin chain. These data establish that native KIF consist of heterodimer coiled-coil molecules. Furthermore, heterodimers are much more stable than homodimers and are the favored form of association in solution. However, some homodimers (10-30% of total) always form after dissolution in concentrated urea and can be assimilated into KIF during reassembly in vitro. The isolation of alpha-helix-enriched dimer particles from the 2B region of the rod domains upon limited proteolysis confirmed the presence of mostly heterodimer and some homodimer molecules in reassembled KIF. These properties of keratin chains in urea solutions hereby clarify a number of conflicting reports in the literature concerning the composition of the coiled-coil molecule. The presence of some homo-oligomeric species in reassembled KIF correlates with earlier observations of polymorphism as well as stoichiometry." @default.
• W1485697910 created "2016-06-24" @default.
• W1485697910 creator A5090122914 @default.
• W1485697910 date "1990-05-01" @default.
• W1485697910 modified "2023-10-18" @default.
• W1485697910 title "The two-chain coiled-coil molecule of native epidermal keratin intermediate filaments is a type I-type II heterodimer." @default.
• W1485697910 cites W1001811995 @default.
• W1485697910 cites W1429171507 @default.
• W1485697910 cites W1506526502 @default.
• W1485697910 cites W1535662492 @default.
• W1485697910 cites W1544906997 @default.
• W1485697910 cites W1557398645 @default.
• W1485697910 cites W1559678336 @default.
• W1485697910 cites W1773698728 @default.
• W1485697910 cites W1941328510 @default.
• W1485697910 cites W1964442439 @default.
• W1485697910 cites W1969779303 @default.
• W1485697910 cites W1974457318 @default.
• W1485697910 cites W1975287171 @default.
• W1485697910 cites W1981427949 @default.
• W1485697910 cites W1984774455 @default.
• W1485697910 cites W1985534974 @default.
• W1485697910 cites W1990949661 @default.
• W1485697910 cites W1999611715 @default.
• W1485697910 cites W2000980346 @default.
• W1485697910 cites W2011770798 @default.
• W1485697910 cites W2013704122 @default.
• W1485697910 cites W2014802721 @default.
• W1485697910 cites W2016693266 @default.
• W1485697910 cites W2023913286 @default.
• W1485697910 cites W2026023235 @default.
• W1485697910 cites W2027423583 @default.
• W1485697910 cites W2035776517 @default.
• W1485697910 cites W2038721717 @default.
• W1485697910 cites W2038854083 @default.
• W1485697910 cites W2041711976 @default.
• W1485697910 cites W2041852399 @default.
• W1485697910 cites W2053487132 @default.
• W1485697910 cites W2057395131 @default.
• W1485697910 cites W2066390328 @default.
• W1485697910 cites W2067259243 @default.
• W1485697910 cites W2069860300 @default.
• W1485697910 cites W2081528773 @default.
• W1485697910 cites W2081743971 @default.
• W1485697910 cites W2085296969 @default.
• W1485697910 cites W2087497884 @default.
• W1485697910 cites W2087985795 @default.
• W1485697910 cites W2089433310 @default.
• W1485697910 cites W2090760822 @default.
• W1485697910 cites W2094659932 @default.
• W1485697910 cites W2119297613 @default.
• W1485697910 cites W2130868634 @default.
• W1485697910 cites W2151860884 @default.
• W1485697910 cites W2160190742 @default.
• W1485697910 cites W2167812799 @default.
• W1485697910 cites W2328293760 @default.
• W1485697910 cites W2338991023 @default.
• W1485697910 cites W2911696842 @default.
• W1485697910 doi "https://doi.org/10.1016/s0021-9258(19)38954-9" @default.
• W1485697910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1692836" @default.
• W1485697910 hasPublicationYear "1990" @default.
• W1485697910 type Work @default.
• W1485697910 sameAs 1485697910 @default.
• W1485697910 citedByCount "188" @default.
• W1485697910 countsByYear W14856979102012 @default.
• W1485697910 countsByYear W14856979102013 @default.
• W1485697910 countsByYear W14856979102014 @default.
• W1485697910 countsByYear W14856979102015 @default.
• W1485697910 countsByYear W14856979102016 @default.
• W1485697910 countsByYear W14856979102017 @default.
• W1485697910 countsByYear W14856979102018 @default.
• W1485697910 countsByYear W14856979102019 @default.
• W1485697910 countsByYear W14856979102020 @default.
• W1485697910 countsByYear W14856979102021 @default.
• W1485697910 countsByYear W14856979102022 @default.
• W1485697910 countsByYear W14856979102023 @default.
• W1485697910 crossrefType "journal-article" @default.
• W1485697910 hasAuthorship W1485697910A5090122914 @default.
• W1485697910 hasBestOaLocation W14856979101 @default.
• W1485697910 hasConcept C121332964 @default.
• W1485697910 hasConcept C12554922 @default.
• W1485697910 hasConcept C1276947 @default.
• W1485697910 hasConcept C142669718 @default.
• W1485697910 hasConcept C1491633281 @default.
• W1485697910 hasConcept C178790620 @default.
• W1485697910 hasConcept C185592680 @default.
• W1485697910 hasConcept C18903297 @default.
• W1485697910 hasConcept C199185054 @default.
• W1485697910 hasConcept C2777299769 @default.
• W1485697910 hasConcept C2993989360 @default.
• W1485697910 hasConcept C32909587 @default.
• W1485697910 hasConcept C515207424 @default.
• W1485697910 hasConcept C54355233 @default.
• W1485697910 hasConcept C55493867 @default.
• W1485697910 hasConcept C55988834 @default.
• W1485697910 hasConcept C78383274 @default.
• W1485697910 hasConcept C8010536 @default.
• W1485697910 hasConcept C81241287 @default.

• next