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• W148624069 abstract "mRNA display is a powerful in vitro selection technique that can be applied toward the identification of peptides or proteins with desired properties. The physical conjugation between a protein and its own RNA presents unique challenges in manipulating the displayed proteins in an RNase-free environment. This protocol outlines the generation of synthetic peptide and natural proteome libraries as well as the steps required for generation of mRNA-protein fusion libraries, in vitro selection, and regeneration of the selected sequences. The selection procedures for the identification of Ca(2+)-dependent, calmodulin-binding proteins from synthetic peptide and natural proteome libraries are presented." @default.
• W148624069 created "2016-06-24" @default.
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• W148624069 date "2011-10-20" @default.
• W148624069 modified "2023-10-16" @default.
• W148624069 title "mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries" @default.
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• W148624069 doi "https://doi.org/10.1007/978-1-61779-379-0_16" @default.
• W148624069 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4259390" @default.
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