Matches in SemOpenAlex for { <https://semopenalex.org/work/W1486321123> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1486321123 endingPage "12" @default.
- W1486321123 startingPage "1" @default.
- W1486321123 abstract "Starting with one strand of the 40-bp synthetic operator (Sadler et al., 1978), we have constructed and cloned a 66-bp, palindromic DNA segment with the following sequence (Formula: see text), where the horizontal arrows indicate the locations of the two 21-bp core operator sequences in this segment and the vertical arrow designates the dyad axis of symmetry. Upon denaturation and rapid renaturation, each strand of this fragment forms a hairpin molecule still retaining an EcoRI cohesive end. Two hairpin molecules can be joined with T4 DNA ligase to form a duplex DNA molecule having no ends (dumbbell form A). Denaturation and rapid renaturation of dumbbell A yields a mixture of two dumbbell forms: dumbbell A which is a substrate for Eco RI, and a new form, dumbbell B, which is not a substrate. Each of the conformations of this DNA fragment have been purified and all are active in binding lactose repressor in vitro." @default.
- W1486321123 created "2016-06-24" @default.
- W1486321123 creator A5003531769 @default.
- W1486321123 creator A5029075668 @default.
- W1486321123 date "1981-01-01" @default.
- W1486321123 modified "2023-09-27" @default.
- W1486321123 title "Variants of a cloned synthetic lactose operator" @default.
- W1486321123 cites W1992042701 @default.
- W1486321123 cites W2001452629 @default.
- W1486321123 cites W2012104867 @default.
- W1486321123 cites W2017953423 @default.
- W1486321123 cites W2021753888 @default.
- W1486321123 cites W2032067706 @default.
- W1486321123 cites W2071965573 @default.
- W1486321123 cites W2077914787 @default.
- W1486321123 cites W2084106399 @default.
- W1486321123 cites W2088705097 @default.
- W1486321123 cites W2094671300 @default.
- W1486321123 cites W2100878960 @default.
- W1486321123 cites W2161003434 @default.
- W1486321123 doi "https://doi.org/10.1016/0378-1119(81)90038-x" @default.
- W1486321123 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7016667" @default.
- W1486321123 hasPublicationYear "1981" @default.
- W1486321123 type Work @default.
- W1486321123 sameAs 1486321123 @default.
- W1486321123 citedByCount "31" @default.
- W1486321123 crossrefType "journal-article" @default.
- W1486321123 hasAuthorship W1486321123A5003531769 @default.
- W1486321123 hasAuthorship W1486321123A5029075668 @default.
- W1486321123 hasConcept C113629113 @default.
- W1486321123 hasConcept C12554922 @default.
- W1486321123 hasConcept C128319531 @default.
- W1486321123 hasConcept C130123407 @default.
- W1486321123 hasConcept C153911025 @default.
- W1486321123 hasConcept C170344550 @default.
- W1486321123 hasConcept C175114707 @default.
- W1486321123 hasConcept C185592680 @default.
- W1486321123 hasConcept C22744801 @default.
- W1486321123 hasConcept C2776546559 @default.
- W1486321123 hasConcept C42407357 @default.
- W1486321123 hasConcept C54355233 @default.
- W1486321123 hasConcept C552990157 @default.
- W1486321123 hasConcept C71240020 @default.
- W1486321123 hasConcept C86803240 @default.
- W1486321123 hasConcept C99611785 @default.
- W1486321123 hasConceptScore W1486321123C113629113 @default.
- W1486321123 hasConceptScore W1486321123C12554922 @default.
- W1486321123 hasConceptScore W1486321123C128319531 @default.
- W1486321123 hasConceptScore W1486321123C130123407 @default.
- W1486321123 hasConceptScore W1486321123C153911025 @default.
- W1486321123 hasConceptScore W1486321123C170344550 @default.
- W1486321123 hasConceptScore W1486321123C175114707 @default.
- W1486321123 hasConceptScore W1486321123C185592680 @default.
- W1486321123 hasConceptScore W1486321123C22744801 @default.
- W1486321123 hasConceptScore W1486321123C2776546559 @default.
- W1486321123 hasConceptScore W1486321123C42407357 @default.
- W1486321123 hasConceptScore W1486321123C54355233 @default.
- W1486321123 hasConceptScore W1486321123C552990157 @default.
- W1486321123 hasConceptScore W1486321123C71240020 @default.
- W1486321123 hasConceptScore W1486321123C86803240 @default.
- W1486321123 hasConceptScore W1486321123C99611785 @default.
- W1486321123 hasIssue "1" @default.
- W1486321123 hasLocation W14863211231 @default.
- W1486321123 hasLocation W14863211232 @default.
- W1486321123 hasOpenAccess W1486321123 @default.
- W1486321123 hasPrimaryLocation W14863211231 @default.
- W1486321123 hasRelatedWork W114656792 @default.
- W1486321123 hasRelatedWork W1486321123 @default.
- W1486321123 hasRelatedWork W1969058261 @default.
- W1486321123 hasRelatedWork W1983947875 @default.
- W1486321123 hasRelatedWork W2027174789 @default.
- W1486321123 hasRelatedWork W2054176944 @default.
- W1486321123 hasRelatedWork W2094761008 @default.
- W1486321123 hasRelatedWork W2145381472 @default.
- W1486321123 hasRelatedWork W2151129454 @default.
- W1486321123 hasRelatedWork W2321080702 @default.
- W1486321123 hasVolume "13" @default.
- W1486321123 isParatext "false" @default.
- W1486321123 isRetracted "false" @default.
- W1486321123 magId "1486321123" @default.
- W1486321123 workType "article" @default.