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- W1486329219 abstract "以培养的原发性肝癌细胞HepG2和正常肝细胞L02为研究对象，采用细胞裂解液提取总蛋白，用PNGase F 酶解释放 N -糖链，以微晶纤维素柱结合石墨碳柱纯化分离 N -糖链，通过电喷雾电离质谱（ESI-MS）和串联质谱（MS/MS）对 N -糖链进行序列鉴定，以 β -环糊精为内标对2种细胞系的 N -糖链进行了定量比较分析. 结果表明，在肝癌细胞系HepG2和正常细胞系L02中共检测到26种 N -糖链，与L02相比，HepG2的大多数高甘露糖型糖链、唾液酸化糖链和岩藻糖基化糖链的数量都明显升高，其中有15种糖链在数量上具有极显著性差异（ p p N -糖链的表达特点及发现早期肝癌糖链标志物提供了参考." @default.
- W1486329219 created "2016-06-24" @default.
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- W1486329219 date "2013-12-11" @default.
- W1486329219 modified "2023-09-23" @default.
- W1486329219 title "基于电喷雾电离质谱的人肝癌细胞HepG2与正常肝细胞L02的 N -糖链的定性定量比较" @default.
- W1486329219 doi "https://doi.org/10.7503/cjcu20130960" @default.
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