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- W1486359207 abstract "ABSTRACT Introduction The main chemical constituents of Caesalpinia sappan are homoisoflavonoids. Conventional column chromatographic techniques used for isolation of this type of compounds are tedious, time‐consuming and waste solvents. High‐speed counter‐current chromatography (HSCCC) could be a suitable alternative for the enrichment and purification of these target compounds from traditional Chinese medicine (TCM). Objective To establish a method to isolate four homoisoflavonoids in one‐step separation from C. sappan by HSCCC. Methodology The crude extract of C. sappan was fractionated by HSCCC using a two‐phase solvent system consisting of chloroform–methanol–water (4:3:2, v/v/v). The separation conditions were: flow rate, 1.0 mL/min; revolution speed, 900 rpm; detection wavelength, 280 nm; separation temperature, 25 °C; sample size, 120 mg crude sample dissolved in a mixture of the upper and lower phases (10 mL each). The retention of the stationary phase was 83%. Results Five milligrams of 3'‐deoxysappanol, 8 mg of 3‐deoxysappanone B, 20 mg of 4‐ O ‐methylsappanol and 18 mg of brazilin were obtained in one‐step separation from 120 mg of an ethyl acetate extracted fraction of C. sappan . Their purities were 99%, 97%, 90% and 85% by HPLC analysis. The mean recoveries of the four compounds were 83%, 86%, 93% and 85%, respectively. Conclusion The study has shown that HSCCC is effective for the separation and enrichment of the target compounds at a large scale. Copyright © 2011 John Wiley & Sons, Ltd." @default.
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- W1486359207 date "2011-08-18" @default.
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- W1486359207 title "Separation of Four Homoisoflavonoids from <i>Caesalpinia sappan</i> by High-speed Counter-current Chromatography" @default.
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- W1486359207 doi "https://doi.org/10.1002/pca.1347" @default.
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