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- W1486372428 abstract "Publisher Summary This chapter analyzes the transcription of antitermination in lambdoid phages encoding toxin genes. Lambdoid phages carry the genes encoding Shiga toxin (Stx A and B): most commonly, the genes encoding the two subunits of either the stx1 or stx2 genes. The two best-studied stx-carrying phages are Stx1 phage H-19B and Stx2 phage 933W. Four methods of analysis were used to determine whether H-19B has an antitermination system similar to that of λ and assess its specific mechanism of action: DNA sequence determination of regions of the H-19B genome that may play a role in antitermination based on studies of λ N-mediated antitermination; growth of H-19B in E. coli mutants defective for λ antitermination; construction and analysis of H-19B phage mutants; and reporter constructs designed to study antitermination mediated by the H-19B N protein. Analysis of H-19B Q-mediated antitermination involved similar methods as those employed in analysis of N-mediated antitermination: sequence determination of the late regulatory region of H-19B, which includes the Q gene, the Prʹ promoter with its associated qut site, and the t R ʹ terminator, as well as stx and lysis genes; phage mutants; and reporter–terminator constructs to measure factors required for antitermination at downstream terminators." @default.
- W1486372428 created "2016-06-24" @default.
- W1486372428 creator A5030647989 @default.
- W1486372428 creator A5033597379 @default.
- W1486372428 date "2003-01-01" @default.
- W1486372428 modified "2023-09-26" @default.
- W1486372428 title "Analyzing Transcription Antitermination in Lambdoid Phages Encoding Toxin Genes" @default.
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- W1486372428 doi "https://doi.org/10.1016/s0076-6879(03)71032-5" @default.
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