FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1486765397> ?p ?o ?g. }

• W1486765397 endingPage "100" @default.
• W1486765397 startingPage "85" @default.
• W1486765397 abstract "Scope High-content imaging (HCI) plays a pivotal role in high-throughput screening (HTS) of biological responses to biomaterials. This chapter will give a brief introduction to its basic principles by explaining the most commonly used imaging techniques, describing the general structure of an image analysis pipeline and providing a summary of available HCI software. Special emphasis will be devoted to the initial steps of image analysis such as image correction, segmentation and feature extraction. Additionally we include a brief overview of relevant literature and description of promising new tools for HCI. Finally, two classic experiments will be described, which use state-of-the-art HCI in biomaterial science by experts in the field. Origins of high-content imaging The first person to observe micro-organisms with a microscopic device was the Dutchman Antoni van Leeuwenhoek (1), who was able to make and polish tiny lenses of high curvature which were the forerunners of the modern microscope. Robert Hooke, the English father of microscopy, later improved on the design and confirmed van Leeuwenhoek’s findings. Since then, light microscopy has evolved into its modern incarnation. The first use of fluorescence microscopy in biology was in 1881 when the bacteriologist Paul Ehrlich used fluorescin to observe the aqueous humour in the eye. The first immuno-staining was performed in 1950 by Melvin Kaplan (2). Green fluorescent protein (GFP) was the first fluorescent protein used in biology. It was isolated from jellyfish in 1961 by Shimomura and co-workers, and it was cloned by Prisher in 1992. The ability either to use antibody conjugated fluorophores or to clone GFP into chimaeric proteins led to the implementation of the fluorescence microscope in modern biology. High-content imaging (HCI) is the automation of fluorescence microscopy whereby the manual interpretation of images is replaced by computer algorithms." @default.
• W1486765397 created "2016-06-24" @default.
• W1486765397 creator A5000009187 @default.
• W1486765397 creator A5004455575 @default.
• W1486765397 creator A5004498168 @default.
• W1486765397 creator A5006826835 @default.
• W1486765397 creator A5015875199 @default.
• W1486765397 creator A5065178874 @default.
• W1486765397 creator A5087750619 @default.
• W1486765397 date "2013-04-23" @default.
• W1486765397 modified "2023-10-02" @default.
• W1486765397 title "High-content imaging" @default.
• W1486765397 cites W1264459389 @default.
• W1486765397 cites W1524205567 @default.
• W1486765397 cites W1905439276 @default.
• W1486765397 cites W1972544340 @default.
• W1486765397 cites W1973194576 @default.
• W1486765397 cites W1985700335 @default.
• W1486765397 cites W1989298707 @default.
• W1486765397 cites W1997529300 @default.
• W1486765397 cites W2000151990 @default.
• W1486765397 cites W2000785720 @default.
• W1486765397 cites W2015966835 @default.
• W1486765397 cites W2015979204 @default.
• W1486765397 cites W2025064029 @default.
• W1486765397 cites W2034045954 @default.
• W1486765397 cites W2037736150 @default.
• W1486765397 cites W2039559580 @default.
• W1486765397 cites W2042094955 @default.
• W1486765397 cites W2046588282 @default.
• W1486765397 cites W2047961352 @default.
• W1486765397 cites W2050794411 @default.
• W1486765397 cites W2057909496 @default.
• W1486765397 cites W2058366604 @default.
• W1486765397 cites W2061218357 @default.
• W1486765397 cites W2065915163 @default.
• W1486765397 cites W2067813822 @default.
• W1486765397 cites W2075744820 @default.
• W1486765397 cites W2086944387 @default.
• W1486765397 cites W2088139400 @default.
• W1486765397 cites W2098295452 @default.
• W1486765397 cites W2100117216 @default.
• W1486765397 cites W2107554012 @default.
• W1486765397 cites W2113769434 @default.
• W1486765397 cites W2130561514 @default.
• W1486765397 cites W2135476182 @default.
• W1486765397 cites W2138825607 @default.
• W1486765397 cites W2140201853 @default.
• W1486765397 cites W2147163301 @default.
• W1486765397 cites W2163886028 @default.
• W1486765397 cites W2164765928 @default.
• W1486765397 cites W2168867052 @default.
• W1486765397 doi "https://doi.org/10.1017/cbo9781139061414.007" @default.
• W1486765397 hasPublicationYear "2013" @default.
• W1486765397 type Work @default.
• W1486765397 sameAs 1486765397 @default.
• W1486765397 citedByCount "0" @default.
• W1486765397 crossrefType "book-chapter" @default.
• W1486765397 hasAuthorship W1486765397A5000009187 @default.
• W1486765397 hasAuthorship W1486765397A5004455575 @default.
• W1486765397 hasAuthorship W1486765397A5004498168 @default.
• W1486765397 hasAuthorship W1486765397A5006826835 @default.
• W1486765397 hasAuthorship W1486765397A5015875199 @default.
• W1486765397 hasAuthorship W1486765397A5065178874 @default.
• W1486765397 hasAuthorship W1486765397A5087750619 @default.
• W1486765397 hasConcept C120665830 @default.
• W1486765397 hasConcept C121332964 @default.
• W1486765397 hasConcept C147080431 @default.
• W1486765397 hasConcept C171250308 @default.
• W1486765397 hasConcept C185592680 @default.
• W1486765397 hasConcept C192562407 @default.
• W1486765397 hasConcept C41008148 @default.
• W1486765397 hasConceptScore W1486765397C120665830 @default.
• W1486765397 hasConceptScore W1486765397C121332964 @default.
• W1486765397 hasConceptScore W1486765397C147080431 @default.
• W1486765397 hasConceptScore W1486765397C171250308 @default.
• W1486765397 hasConceptScore W1486765397C185592680 @default.
• W1486765397 hasConceptScore W1486765397C192562407 @default.
• W1486765397 hasConceptScore W1486765397C41008148 @default.
• W1486765397 hasLocation W14867653971 @default.
• W1486765397 hasOpenAccess W1486765397 @default.
• W1486765397 hasPrimaryLocation W14867653971 @default.
• W1486765397 hasRelatedWork W1853569464 @default.
• W1486765397 hasRelatedWork W1968300924 @default.
• W1486765397 hasRelatedWork W1983665434 @default.
• W1486765397 hasRelatedWork W2006292456 @default.
• W1486765397 hasRelatedWork W2024825881 @default.
• W1486765397 hasRelatedWork W2030574742 @default.
• W1486765397 hasRelatedWork W2038134596 @default.
• W1486765397 hasRelatedWork W2047845259 @default.
• W1486765397 hasRelatedWork W2066799353 @default.
• W1486765397 hasRelatedWork W2096521567 @default.
• W1486765397 hasRelatedWork W2133462928 @default.
• W1486765397 hasRelatedWork W2139780115 @default.
• W1486765397 hasRelatedWork W2148440363 @default.
• W1486765397 hasRelatedWork W2200979507 @default.
• W1486765397 hasRelatedWork W2240699928 @default.
• W1486765397 hasRelatedWork W2283163132 @default.

• next