Matches in SemOpenAlex for { <https://semopenalex.org/work/W1487108001> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W1487108001 abstract "High Ca2+ scores assessed by computed tomography in the lower limbs of patients with peripheral artery disease are associated with a greater risk of amputation and all-cause mortality. Direct assessment of intracellular Ca2+ handling in peripheral vascular smooth muscle cells (VSM) of humans likely to undergo an amputation is unknown due to the invasiveness of such measures. We directly characterized intracellular VSM Ca2+ handling in arteries from amputated human limbs stratified by the number of diagnosed cardiovascular (CV) risk factors (<2 vs >6 CV risk factors). VSM were enzymatically isolated from peripheral arteries and were digitally imaged using the fluorescent intracellular Ca2+ indicator, fura-2. Cells were exposed to 80 mM K+ in physiologic Ca2+ to assess Ca2+ entry through voltage-gated Ca2+ channels (VGCC). Caffeine was used in the presence and absence of extracellular Ca2+ to release intracellular Ca2+ from the sarcoplasmic reticulum (SR). VSM from patients with >6 CV risk factors had elevated basal cytosolic Ca2+ (0.76±0.01 vs 0.72±0.01 F360/F380) and greater Ca2+ entry through VGCC (0.08±0.01 vs 0.05±0.00 ΔF360/F380) vs patients with <2 CV risk factors. In the absence of extracellular Ca2+, the SR Ca2+ store release was decreased in VSM in patients with >6 CV risk factors (0.16±0.02 vs 0.22±0.01 F360/F380). However, this decrease was absent in the presence of extracellular Ca2+, suggesting elevated Ca2+-activated Ca2+ influx with more CV risk factors. Taken together, these data suggest that changes in VGCC, SR Ca2+ stores, and Ca2+-activated Ca2+ influx act to elevate cytosolic Ca2+ in VSM of amputees with more CV risk factors. NIH T32 HL079995, Health-IUSM Strategic Research Initiative" @default.
- W1487108001 created "2016-06-24" @default.
- W1487108001 creator A5049007023 @default.
- W1487108001 creator A5068245451 @default.
- W1487108001 date "2015-04-01" @default.
- W1487108001 modified "2023-10-14" @default.
- W1487108001 title "Characterization of Intracellular Ca 2+ Handling in Vascular Smooth Muscle Cells Isolated from Amputated Human Limbs" @default.
- W1487108001 doi "https://doi.org/10.1096/fasebj.29.1_supplement.638.3" @default.
- W1487108001 hasPublicationYear "2015" @default.
- W1487108001 type Work @default.
- W1487108001 sameAs 1487108001 @default.
- W1487108001 citedByCount "0" @default.
- W1487108001 crossrefType "journal-article" @default.
- W1487108001 hasAuthorship W1487108001A5049007023 @default.
- W1487108001 hasAuthorship W1487108001A5068245451 @default.
- W1487108001 hasConcept C126322002 @default.
- W1487108001 hasConcept C134018914 @default.
- W1487108001 hasConcept C158617107 @default.
- W1487108001 hasConcept C164705383 @default.
- W1487108001 hasConcept C181199279 @default.
- W1487108001 hasConcept C185592680 @default.
- W1487108001 hasConcept C2778024521 @default.
- W1487108001 hasConcept C2778470358 @default.
- W1487108001 hasConcept C2778500429 @default.
- W1487108001 hasConcept C2779306644 @default.
- W1487108001 hasConcept C2779395532 @default.
- W1487108001 hasConcept C28406088 @default.
- W1487108001 hasConcept C2992686903 @default.
- W1487108001 hasConcept C46762472 @default.
- W1487108001 hasConcept C519063684 @default.
- W1487108001 hasConcept C55493867 @default.
- W1487108001 hasConcept C71924100 @default.
- W1487108001 hasConcept C79879829 @default.
- W1487108001 hasConcept C98539663 @default.
- W1487108001 hasConceptScore W1487108001C126322002 @default.
- W1487108001 hasConceptScore W1487108001C134018914 @default.
- W1487108001 hasConceptScore W1487108001C158617107 @default.
- W1487108001 hasConceptScore W1487108001C164705383 @default.
- W1487108001 hasConceptScore W1487108001C181199279 @default.
- W1487108001 hasConceptScore W1487108001C185592680 @default.
- W1487108001 hasConceptScore W1487108001C2778024521 @default.
- W1487108001 hasConceptScore W1487108001C2778470358 @default.
- W1487108001 hasConceptScore W1487108001C2778500429 @default.
- W1487108001 hasConceptScore W1487108001C2779306644 @default.
- W1487108001 hasConceptScore W1487108001C2779395532 @default.
- W1487108001 hasConceptScore W1487108001C28406088 @default.
- W1487108001 hasConceptScore W1487108001C2992686903 @default.
- W1487108001 hasConceptScore W1487108001C46762472 @default.
- W1487108001 hasConceptScore W1487108001C519063684 @default.
- W1487108001 hasConceptScore W1487108001C55493867 @default.
- W1487108001 hasConceptScore W1487108001C71924100 @default.
- W1487108001 hasConceptScore W1487108001C79879829 @default.
- W1487108001 hasConceptScore W1487108001C98539663 @default.
- W1487108001 hasFunder F4320332161 @default.
- W1487108001 hasIssue "S1" @default.
- W1487108001 hasLocation W14871080011 @default.
- W1487108001 hasOpenAccess W1487108001 @default.
- W1487108001 hasPrimaryLocation W14871080011 @default.
- W1487108001 hasRelatedWork W1968681020 @default.
- W1487108001 hasRelatedWork W1993528131 @default.
- W1487108001 hasRelatedWork W1993707289 @default.
- W1487108001 hasRelatedWork W2021727957 @default.
- W1487108001 hasRelatedWork W2026063665 @default.
- W1487108001 hasRelatedWork W2072105226 @default.
- W1487108001 hasRelatedWork W2074851486 @default.
- W1487108001 hasRelatedWork W2318749927 @default.
- W1487108001 hasRelatedWork W2372685958 @default.
- W1487108001 hasRelatedWork W2396661272 @default.
- W1487108001 hasVolume "29" @default.
- W1487108001 isParatext "false" @default.
- W1487108001 isRetracted "false" @default.
- W1487108001 magId "1487108001" @default.
- W1487108001 workType "article" @default.