FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1487753561> ?p ?o ?g. }

• W1487753561 endingPage "184" @default.
• W1487753561 startingPage "129" @default.
• W1487753561 abstract "The pyridoxal-5-phosphate-dependent enzymes (B6 enzymes) that act on amino acid substrates are of multiple evolutionary origin. The numerous common mechanistic features of B6 enzymes thus are not historical traits passed on from a common ancestor enzyme but rather reflect evolutionary or chemical necessities. Family profile analysis of amino acid sequences supported by comparison of the available three-dimensional (3-D) crystal structures indicates that the B6 enzymes known to date belong to four independent evolutionary lineages of homologous (or more precisely paralogous) proteins, of which the alpha family is by far the largest. The alpha family (with aspartate aminotransferase as the prototype enzyme) includes enzymes that catalyze, with several exceptions, transformations of amino acids in which the covalency changes are limited to the same carbon atom that carries the amino group forming the imine linkage with the coenzyme (i.e., Calpha in most cases). Enzymes of the beta family (tryptophan synthase beta as the prototype enzyme) mainly catalyze replacement and elimination reactions at Cbeta. The D-alanine aminotransferase family and the alanine racemase family are the two other independent lineages, both with relatively few member enzymes. The primordial pyridoxal-5-phosphate-dependent enzymes apparently were regio-specific catalysts that first diverged into reaction-specific enzymes and then specialized for substrate specificity. Aminotransferases as well as amino acid decarboxylases are found in two different evolutionary lineages. Comparison of sequences from eukaryotic, archebacterial, and eubacterial species indicates that the functional specialization of most B6 enzymes has occurred already in the universal ancestor cell. The cofactor pyridoxal-5-phosphate must have emerged very early in biological evolution; conceivably, organic cofactors and metal ions were the first biological catalysts. In attempts to stimulate particular steps of molecular evolution, oligonucleotide-directed mutagenesis of active-site residues and directed molecular evolution have been applied to change both the substrate and reaction specificity of existent B6 enzymes. Pyridoxal-5-phosphate-dependent catalytic antibodies were elicited with a screening protocol that applied functional selection criteria as they might have been operative in the evolution of protein-assisted pyridoxal catalysis." @default.
• W1487753561 created "2016-06-24" @default.
• W1487753561 creator A5001258452 @default.
• W1487753561 creator A5087072437 @default.
• W1487753561 date "2006-11-22" @default.
• W1487753561 modified "2023-10-11" @default.
• W1487753561 title "The Molecular Evolution of Pyridoxal-5′-Phosphate-Dependent Enzymes" @default.
• W1487753561 cites W1422803820 @default.
• W1487753561 cites W1481073719 @default.
• W1487753561 cites W1486176644 @default.
• W1487753561 cites W1489182117 @default.
• W1487753561 cites W1492703699 @default.
• W1487753561 cites W1505216348 @default.
• W1487753561 cites W1508515597 @default.
• W1487753561 cites W1512449069 @default.
• W1487753561 cites W1523914622 @default.
• W1487753561 cites W1532657808 @default.
• W1487753561 cites W1542852458 @default.
• W1487753561 cites W1550755354 @default.
• W1487753561 cites W1560698721 @default.
• W1487753561 cites W1561910889 @default.
• W1487753561 cites W1562562461 @default.
• W1487753561 cites W1589282929 @default.
• W1487753561 cites W1590937153 @default.
• W1487753561 cites W1592800434 @default.
• W1487753561 cites W1602077774 @default.
• W1487753561 cites W1605797683 @default.
• W1487753561 cites W1617728059 @default.
• W1487753561 cites W1628606745 @default.
• W1487753561 cites W1654282388 @default.
• W1487753561 cites W1655608294 @default.
• W1487753561 cites W1771251144 @default.
• W1487753561 cites W1773196754 @default.
• W1487753561 cites W179795275 @default.
• W1487753561 cites W1861199552 @default.
• W1487753561 cites W1955968728 @default.
• W1487753561 cites W1966041739 @default.
• W1487753561 cites W1967999247 @default.
• W1487753561 cites W1968063959 @default.
• W1487753561 cites W1968547228 @default.
• W1487753561 cites W1968621693 @default.
• W1487753561 cites W1969245715 @default.
• W1487753561 cites W1970229178 @default.
• W1487753561 cites W1970473208 @default.
• W1487753561 cites W1972151960 @default.
• W1487753561 cites W1973183626 @default.
• W1487753561 cites W1975350891 @default.
• W1487753561 cites W1977571339 @default.
• W1487753561 cites W1977727057 @default.
• W1487753561 cites W1977857718 @default.
• W1487753561 cites W1978353572 @default.
• W1487753561 cites W1979453863 @default.
• W1487753561 cites W1979489154 @default.
• W1487753561 cites W1983558037 @default.
• W1487753561 cites W1984210216 @default.
• W1487753561 cites W1987345363 @default.
• W1487753561 cites W1987802907 @default.
• W1487753561 cites W1987814777 @default.
• W1487753561 cites W1990067685 @default.
• W1487753561 cites W1990258186 @default.
• W1487753561 cites W1994426626 @default.
• W1487753561 cites W1995797307 @default.
• W1487753561 cites W1995945233 @default.
• W1487753561 cites W1996032119 @default.
• W1487753561 cites W1996196679 @default.
• W1487753561 cites W1997115108 @default.
• W1487753561 cites W1998346178 @default.
• W1487753561 cites W2000409240 @default.
• W1487753561 cites W2003300391 @default.
• W1487753561 cites W2003764452 @default.
• W1487753561 cites W2008171507 @default.
• W1487753561 cites W2008511903 @default.
• W1487753561 cites W2009310436 @default.
• W1487753561 cites W2010934068 @default.
• W1487753561 cites W2012036455 @default.
• W1487753561 cites W2013414388 @default.
• W1487753561 cites W2013414898 @default.
• W1487753561 cites W2016243826 @default.
• W1487753561 cites W2016356419 @default.
• W1487753561 cites W2017661263 @default.
• W1487753561 cites W2023759182 @default.
• W1487753561 cites W2028858498 @default.
• W1487753561 cites W2029949059 @default.
• W1487753561 cites W2031145059 @default.
• W1487753561 cites W2031866837 @default.
• W1487753561 cites W2032464095 @default.
• W1487753561 cites W2033719772 @default.
• W1487753561 cites W2034883796 @default.
• W1487753561 cites W2035224458 @default.
• W1487753561 cites W2035943254 @default.
• W1487753561 cites W2036931862 @default.
• W1487753561 cites W2040061286 @default.
• W1487753561 cites W2042494872 @default.
• W1487753561 cites W2042505702 @default.
• W1487753561 cites W2043006564 @default.
• W1487753561 cites W2046822221 @default.
• W1487753561 cites W2047461365 @default.
• W1487753561 cites W2047692305 @default.

• next