Matches in SemOpenAlex for { <https://semopenalex.org/work/W1488219825> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1488219825 endingPage "2164" @default.
- W1488219825 startingPage "2158" @default.
- W1488219825 abstract "A modified cloning procedure was used to obtain large DNA insertions (20 to 30 kb) from Pseudomonas putida NCIB 9816 that expressed polycyclic aromatic hydrocarbon (PAH) transformation activity in Escherichia coli HB101. Four subclones (16 [in both orientations], 12, and 8.5 kb in size) were constructed from the initial clones. Naphthalene, fluorene, and phenanthrene transformations were investigated in these eight NCIB 9816 clones by a simple agar plate assay method, which was developed to detect and identify potential PAH metabolites. Results indicated that the necessary genes encoding the initial ring fission of the three PAHs in E. coli cells are located in an 8.5-kb EcoRI-XhoI portion, but the lower-pathway genes are not present in a 38-kb neighborhood region. These NCIB 9816 clones could transform naphthalene and phenanthrene to salicylic acid and 1-hydroxy-2-naphthoic acid, respectively. With the same clones, fluorene was degraded to 9-hydroxyfluorene, 9-fluorenone, and two unidentified compounds. Genetic similarity between the NAH7 upper-pathway genes and the cloned NCIB 9816 genes was confirmed by Southern blot DNA-DNA hybridization. In spite of this genetic similarity, the abilities of the two clusters to transform multiple PAHs were different. Under our experimental conditions, only the metabolites from naphthalene transformation by the NAH7 clone (pE317) were detected, whereas the NCIB 9816 clones produced metabolites from all three PAHs." @default.
- W1488219825 created "2016-06-24" @default.
- W1488219825 creator A5009291184 @default.
- W1488219825 creator A5046941230 @default.
- W1488219825 creator A5079782542 @default.
- W1488219825 date "1994-04-01" @default.
- W1488219825 modified "2023-10-13" @default.
- W1488219825 title "Metabolism of naphthalene, fluorene, and phenanthrene: preliminary characterization of a cloned gene cluster from Pseudomonas putida NCIB 9816" @default.
- W1488219825 cites W1500963971 @default.
- W1488219825 cites W1510612702 @default.
- W1488219825 cites W1548396112 @default.
- W1488219825 cites W1567154486 @default.
- W1488219825 cites W1592048772 @default.
- W1488219825 cites W1598919556 @default.
- W1488219825 cites W1874558002 @default.
- W1488219825 cites W1925716288 @default.
- W1488219825 cites W1995441874 @default.
- W1488219825 cites W1997562369 @default.
- W1488219825 cites W2025959969 @default.
- W1488219825 cites W2037507614 @default.
- W1488219825 cites W2064598038 @default.
- W1488219825 cites W2065716368 @default.
- W1488219825 cites W2066425727 @default.
- W1488219825 cites W2073755842 @default.
- W1488219825 cites W2139669165 @default.
- W1488219825 cites W2144604686 @default.
- W1488219825 cites W2165340504 @default.
- W1488219825 cites W2187927698 @default.
- W1488219825 cites W4251567340 @default.
- W1488219825 doi "https://doi.org/10.1128/jb.176.8.2158-2164.1994" @default.
- W1488219825 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/205334" @default.
- W1488219825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8157584" @default.
- W1488219825 hasPublicationYear "1994" @default.
- W1488219825 type Work @default.
- W1488219825 sameAs 1488219825 @default.
- W1488219825 citedByCount "102" @default.
- W1488219825 countsByYear W14882198252012 @default.
- W1488219825 countsByYear W14882198252013 @default.
- W1488219825 countsByYear W14882198252014 @default.
- W1488219825 countsByYear W14882198252015 @default.
- W1488219825 countsByYear W14882198252016 @default.
- W1488219825 countsByYear W14882198252017 @default.
- W1488219825 countsByYear W14882198252018 @default.
- W1488219825 countsByYear W14882198252019 @default.
- W1488219825 countsByYear W14882198252020 @default.
- W1488219825 countsByYear W14882198252021 @default.
- W1488219825 countsByYear W14882198252022 @default.
- W1488219825 countsByYear W14882198252023 @default.
- W1488219825 crossrefType "journal-article" @default.
- W1488219825 hasAuthorship W1488219825A5009291184 @default.
- W1488219825 hasAuthorship W1488219825A5046941230 @default.
- W1488219825 hasAuthorship W1488219825A5079782542 @default.
- W1488219825 hasBestOaLocation W14882198251 @default.
- W1488219825 hasConcept C104317684 @default.
- W1488219825 hasConcept C150194340 @default.
- W1488219825 hasConcept C153911025 @default.
- W1488219825 hasConcept C19924922 @default.
- W1488219825 hasConcept C2776891815 @default.
- W1488219825 hasConcept C2780543182 @default.
- W1488219825 hasConcept C55493867 @default.
- W1488219825 hasConcept C86803240 @default.
- W1488219825 hasConcept C87355193 @default.
- W1488219825 hasConceptScore W1488219825C104317684 @default.
- W1488219825 hasConceptScore W1488219825C150194340 @default.
- W1488219825 hasConceptScore W1488219825C153911025 @default.
- W1488219825 hasConceptScore W1488219825C19924922 @default.
- W1488219825 hasConceptScore W1488219825C2776891815 @default.
- W1488219825 hasConceptScore W1488219825C2780543182 @default.
- W1488219825 hasConceptScore W1488219825C55493867 @default.
- W1488219825 hasConceptScore W1488219825C86803240 @default.
- W1488219825 hasConceptScore W1488219825C87355193 @default.
- W1488219825 hasIssue "8" @default.
- W1488219825 hasLocation W14882198251 @default.
- W1488219825 hasLocation W14882198252 @default.
- W1488219825 hasLocation W14882198253 @default.
- W1488219825 hasLocation W14882198254 @default.
- W1488219825 hasOpenAccess W1488219825 @default.
- W1488219825 hasPrimaryLocation W14882198251 @default.
- W1488219825 hasRelatedWork W1488219825 @default.
- W1488219825 hasRelatedWork W1979833452 @default.
- W1488219825 hasRelatedWork W1989639494 @default.
- W1488219825 hasRelatedWork W2002041269 @default.
- W1488219825 hasRelatedWork W2034072637 @default.
- W1488219825 hasRelatedWork W2076771161 @default.
- W1488219825 hasRelatedWork W2077663007 @default.
- W1488219825 hasRelatedWork W2090429921 @default.
- W1488219825 hasRelatedWork W2350366916 @default.
- W1488219825 hasRelatedWork W2362492361 @default.
- W1488219825 hasVolume "176" @default.
- W1488219825 isParatext "false" @default.
- W1488219825 isRetracted "false" @default.
- W1488219825 magId "1488219825" @default.
- W1488219825 workType "article" @default.