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- W1488308068 abstract "The total thiol content of highly pure jack bean urease (urea amidohydrolase, EC 3.5.1.5) has been determined by titration with 5,5′-dithiobis(2-nitrobenzoic acid) in the presence of 6 M guanidinium chloride. Urease contains 15 thioi groups per 96.6-kDa subunit. Coupled with amino acid analysis data, this result establishes that urease contains one cystine disulfide bond per 96.6-kDa subunit. Slow loss of enzymatic activity in the presence of β-mercaptoethanol and oxygen is due to the formation of a mixed disulfide which involves the unique active-site cysteine residue. Enzymatic activity can be fully restored by treatment of inactive urease with 0.1 M dithiothreitol at pH 7.3. Urease is quite stable when stored in 0.05 M sulfite/0.02 M phosphate buffer (pH 7.2, 1 mM in EDTA)." @default.
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- W1488308068 date "1983-02-01" @default.
- W1488308068 modified "2023-10-17" @default.
- W1488308068 title "Jack Bean Urease VI. Determination of thiol and disulfide content" @default.
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- W1488308068 doi "https://doi.org/10.1016/0167-4838(83)90424-7" @default.
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