Matches in SemOpenAlex for { <https://semopenalex.org/work/W1488407312> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1488407312 endingPage "124.4" @default.
- W1488407312 startingPage "124.4" @default.
- W1488407312 abstract "Abstract Autophagy is a process in which normal cellular components that accumulate during growth and differentiation are degraded via the lysosome; it is a survival mechanism that reallocates nutrients from unnecessary processes to more vital processes in the cell. Basal levels of autophagy are usually low but can be up-regulated by numerous stimuli including starvation, physiological stress, pharmacological agents and infections. In addition, autophagy suppression has been associated with cancer, neurodegenerative disorders, infectious diseases and inflammation. During autophagy, cytoplasmic LC3 is processed and recruited to the outer membrane of forming autophagosomes, and cells undergoing autophagy can be identified by visualizing LC3 puncta by immunofluorescence microscopy. While manual microscopy allows visual per-cell identification of autophagy, objective statistical analysis of autophagy can be challenging with this method owing to its time-consuming and generally subjective nature. To overcome these problems, we used the ImgeStreamX imaging cytometry platform, which objectively collects and quantifies statistically large numbers of images per sample, to measure autophagy in K562 cells, a human CML line. In this study, we demonstrate a method for determining the best image-based parameter for quantifying LC3 puncta in these cells, and apply that parameter to large data sets to measure compound-induced autophagy in an objectively and statistically significant manner." @default.
- W1488407312 created "2016-06-24" @default.
- W1488407312 creator A5001596608 @default.
- W1488407312 creator A5013942467 @default.
- W1488407312 creator A5015094640 @default.
- W1488407312 creator A5016152189 @default.
- W1488407312 creator A5028488948 @default.
- W1488407312 creator A5034409221 @default.
- W1488407312 creator A5060154022 @default.
- W1488407312 date "2012-05-01" @default.
- W1488407312 modified "2023-09-27" @default.
- W1488407312 title "A novel method for autophagy analysis using statistical microscopy (124.4)" @default.
- W1488407312 doi "https://doi.org/10.4049/jimmunol.188.supp.124.4" @default.
- W1488407312 hasPublicationYear "2012" @default.
- W1488407312 type Work @default.
- W1488407312 sameAs 1488407312 @default.
- W1488407312 citedByCount "0" @default.
- W1488407312 crossrefType "journal-article" @default.
- W1488407312 hasAuthorship W1488407312A5001596608 @default.
- W1488407312 hasAuthorship W1488407312A5013942467 @default.
- W1488407312 hasAuthorship W1488407312A5015094640 @default.
- W1488407312 hasAuthorship W1488407312A5016152189 @default.
- W1488407312 hasAuthorship W1488407312A5028488948 @default.
- W1488407312 hasAuthorship W1488407312A5034409221 @default.
- W1488407312 hasAuthorship W1488407312A5060154022 @default.
- W1488407312 hasConcept C102568950 @default.
- W1488407312 hasConcept C1491633281 @default.
- W1488407312 hasConcept C171122931 @default.
- W1488407312 hasConcept C181199279 @default.
- W1488407312 hasConcept C190062978 @default.
- W1488407312 hasConcept C190283241 @default.
- W1488407312 hasConcept C203014093 @default.
- W1488407312 hasConcept C203522944 @default.
- W1488407312 hasConcept C2780216420 @default.
- W1488407312 hasConcept C54355233 @default.
- W1488407312 hasConcept C553184892 @default.
- W1488407312 hasConcept C55493867 @default.
- W1488407312 hasConcept C81885089 @default.
- W1488407312 hasConcept C86803240 @default.
- W1488407312 hasConcept C95444343 @default.
- W1488407312 hasConceptScore W1488407312C102568950 @default.
- W1488407312 hasConceptScore W1488407312C1491633281 @default.
- W1488407312 hasConceptScore W1488407312C171122931 @default.
- W1488407312 hasConceptScore W1488407312C181199279 @default.
- W1488407312 hasConceptScore W1488407312C190062978 @default.
- W1488407312 hasConceptScore W1488407312C190283241 @default.
- W1488407312 hasConceptScore W1488407312C203014093 @default.
- W1488407312 hasConceptScore W1488407312C203522944 @default.
- W1488407312 hasConceptScore W1488407312C2780216420 @default.
- W1488407312 hasConceptScore W1488407312C54355233 @default.
- W1488407312 hasConceptScore W1488407312C553184892 @default.
- W1488407312 hasConceptScore W1488407312C55493867 @default.
- W1488407312 hasConceptScore W1488407312C81885089 @default.
- W1488407312 hasConceptScore W1488407312C86803240 @default.
- W1488407312 hasConceptScore W1488407312C95444343 @default.
- W1488407312 hasIssue "1_Supplement" @default.
- W1488407312 hasLocation W14884073121 @default.
- W1488407312 hasOpenAccess W1488407312 @default.
- W1488407312 hasPrimaryLocation W14884073121 @default.
- W1488407312 hasRelatedWork W2017420757 @default.
- W1488407312 hasRelatedWork W2025732672 @default.
- W1488407312 hasRelatedWork W2043039094 @default.
- W1488407312 hasRelatedWork W2892514092 @default.
- W1488407312 hasRelatedWork W2975967821 @default.
- W1488407312 hasRelatedWork W2998793527 @default.
- W1488407312 hasRelatedWork W3031427514 @default.
- W1488407312 hasRelatedWork W3036214510 @default.
- W1488407312 hasRelatedWork W3115150918 @default.
- W1488407312 hasRelatedWork W4366602043 @default.
- W1488407312 hasVolume "188" @default.
- W1488407312 isParatext "false" @default.
- W1488407312 isRetracted "false" @default.
- W1488407312 magId "1488407312" @default.
- W1488407312 workType "article" @default.