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- W1488653535 abstract "A simplified procedure for isolation of specific mRNA using Staphylococcus aureus protein A immunoadsorbent chromatography has been developed. This procedure has been applied to the mRNA encoding Trypanosoma brucei variable surface antigen. Trypanosome polyribosomes were reacted with antibodies isolated from an anti-variable antigen-specific serum by protein A-Sepharose column chromatography. Antibody-bound variable antigen-synthesizing polyribosomes were then separated from unbound polyribosomes also by protein A-Sepharose column chromatography. This simple protocol gave a greater than 50% yield of variable antigen-specific mRNA which appeared to be very highly purified as determined by translation in an mRNA-dependent reticulocyte lysate assay. The mRNAs encoding three different T. brucei surface antigens have been purified. The procedure described here should be useful in purification of other mRNAs." @default.
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- W1488653535 date "1981-02-01" @default.
- W1488653535 modified "2023-10-18" @default.
- W1488653535 title "An immunochemical method for mRNA purification. Application to messenger RNA encoding trypanosome variable surface antigen." @default.
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- W1488653535 doi "https://doi.org/10.1016/s0021-9258(19)69825-x" @default.
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