Matches in SemOpenAlex for { <https://semopenalex.org/work/W1488847709> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W1488847709 endingPage "5434" @default.
- W1488847709 startingPage "5429" @default.
- W1488847709 abstract "We introduce two new, rapid procedures. One is specifically designed for isolating alpha-actinin from skeletal and the other for isolating alpha-actinin from smooth muscle. Approximately 20 mg of greater than 95% pure alpha-actinin can be obtained/100 g of ground chicken pectoral muscle in just 4 days. The smooth muscle protocol yields 2.7 mg of greater than 99% pure alpha-actinin/100 g of ground gizzard after just 5 days. Differences in protein contaminants and in the extractability of alpha-actinin necessitated the development of separate isolation procedures for the two muscle types. Antibody prepared against the purified gizzard alpha-actinin reacted with alpha-actinin from skeletal, cardiac, and smooth muscle in immunodiffusion. Anti-alpha-actinin reacted only with alpha-actinin from crude extracts of skeletal and smooth muscle on Staph A gels. Anti-alpha-actinin stained Z-bands from skeletal muscle in indirect immunofluorescence microscopy and stress fibers from baby hamster kidney fibroblasts and mouse mammary epithelial cells in the characteristic punctate pattern observed by other workers (Lazarides, E., and Burridge, K. (1975) Cell 6, 289-298). These two methods for purifying alpha-actinin from skeletal and smooth muscle represent a significant improvement over that published previously." @default.
- W1488847709 created "2016-06-24" @default.
- W1488847709 creator A5006180676 @default.
- W1488847709 creator A5022487671 @default.
- W1488847709 date "1980-06-01" @default.
- W1488847709 modified "2023-10-18" @default.
- W1488847709 title "New, rapid methods for purifying alpha-actinin from chicken gizzard and chicken pectoral muscle." @default.
- W1488847709 cites W1497181681 @default.
- W1488847709 cites W1544245820 @default.
- W1488847709 cites W1570776822 @default.
- W1488847709 cites W1581804688 @default.
- W1488847709 cites W1975145133 @default.
- W1488847709 cites W1997121024 @default.
- W1488847709 cites W2024499829 @default.
- W1488847709 cites W2037294635 @default.
- W1488847709 cites W2044363101 @default.
- W1488847709 cites W2048553466 @default.
- W1488847709 cites W2062099855 @default.
- W1488847709 cites W2063639703 @default.
- W1488847709 cites W2100837269 @default.
- W1488847709 cites W2108569275 @default.
- W1488847709 cites W2159049703 @default.
- W1488847709 cites W2418850862 @default.
- W1488847709 cites W4313377000 @default.
- W1488847709 cites W4378744526 @default.
- W1488847709 cites W63309837 @default.
- W1488847709 doi "https://doi.org/10.1016/s0021-9258(19)70804-7" @default.
- W1488847709 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6768740" @default.
- W1488847709 hasPublicationYear "1980" @default.
- W1488847709 type Work @default.
- W1488847709 sameAs 1488847709 @default.
- W1488847709 citedByCount "22" @default.
- W1488847709 countsByYear W14888477092014 @default.
- W1488847709 crossrefType "journal-article" @default.
- W1488847709 hasAuthorship W1488847709A5006180676 @default.
- W1488847709 hasAuthorship W1488847709A5022487671 @default.
- W1488847709 hasBestOaLocation W14888477091 @default.
- W1488847709 hasConcept C105702510 @default.
- W1488847709 hasConcept C151730666 @default.
- W1488847709 hasConcept C185592680 @default.
- W1488847709 hasConcept C206332053 @default.
- W1488847709 hasConcept C2909720742 @default.
- W1488847709 hasConcept C86803240 @default.
- W1488847709 hasConcept C95444343 @default.
- W1488847709 hasConceptScore W1488847709C105702510 @default.
- W1488847709 hasConceptScore W1488847709C151730666 @default.
- W1488847709 hasConceptScore W1488847709C185592680 @default.
- W1488847709 hasConceptScore W1488847709C206332053 @default.
- W1488847709 hasConceptScore W1488847709C2909720742 @default.
- W1488847709 hasConceptScore W1488847709C86803240 @default.
- W1488847709 hasConceptScore W1488847709C95444343 @default.
- W1488847709 hasIssue "11" @default.
- W1488847709 hasLocation W14888477091 @default.
- W1488847709 hasOpenAccess W1488847709 @default.
- W1488847709 hasPrimaryLocation W14888477091 @default.
- W1488847709 hasRelatedWork W1488847709 @default.
- W1488847709 hasRelatedWork W1964333641 @default.
- W1488847709 hasRelatedWork W2004236045 @default.
- W1488847709 hasRelatedWork W2042571285 @default.
- W1488847709 hasRelatedWork W2082339727 @default.
- W1488847709 hasRelatedWork W2093730790 @default.
- W1488847709 hasRelatedWork W2139790161 @default.
- W1488847709 hasRelatedWork W2326838188 @default.
- W1488847709 hasRelatedWork W2328241504 @default.
- W1488847709 hasRelatedWork W2945721103 @default.
- W1488847709 hasVolume "255" @default.
- W1488847709 isParatext "false" @default.
- W1488847709 isRetracted "false" @default.
- W1488847709 magId "1488847709" @default.
- W1488847709 workType "article" @default.