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- W1488922054 abstract "Abstract We summarized the recent work of our group with electrochemistry as the main study method. This chapter was divided into seven sections. 1. The ion-channel behavior of gramicidin in bilayer lipid membrane (BLM) . Gramicidin was incorporated in a BLM. The behavior of the ion channel was studied by cyclic voltammetry. At a very low concentration of gramicidin in a BLM, the channel behavior was controlled by the voltage applied across the membrane. When the voltage is higher than 75 mV, the channel is closing, while lower than 75 mV, the channel is opening, but when the concentration of the gramicidin in the BLMs is high, the channel behavior is changed to voltage-independent. 2. In this section the method of forming a BLM was described, and its character was evaluated by electrochemical methods. 3. The permeation of a BLM regulated by ion . Interaction of lanthanide ions with supported BLMs (s-BLMs) was investigated by cyclic voltammetry (CV) and a.c. impedance spectroscopy. Lanthanide can affect the conformation of s-BLM and make it form some pores through which Fe ( CN ) 6 3 − / 4 − can reach the electrode surface. The interaction ability of three lanthanides with s-BLM follows the sequence: Eu 3+ >Tb 3+ >La 3+ . 4. The interaction of peptide with a s-BLM . We described two kind of peptides interacted with a s-BLM in this section. These peptides include microperoxidase and nisin. The interaction of microperoxidase-11 (MP11) with cationic lipid vesicles of didodecyldimethylammonium bromide induces an α-helical conformation from random coils conformations in solution and then this change makes heme macrocycle more distorted. The binding of MP11 in solution to DDAB vesicles and the ordered structure formation are driven mostly by electrostatic interaction between negatively charged residues in the undecapeptide and positively charged lipid headgroups on the membrane surface. Upon binding to DDAB, its half-peak potential was also changed. Nisin is a positively charged antibacterial peptide which binds to the negatively charged membranes of Gram-positive bacteria. The initial interaction of the peptide with a model membrane of negatively charged dipalmitoylphosphatidylglycerol (DPPG) model lipid membranes was studied by voltammetry and a.c. impedance spectroscopy. Adsorption of nisin into BLM destroyed the insulating capability of BLM, causing membrane resistance to decrease and membrane capacitance to increase. Experimental results suggested that the selective interaction of positively charged nisin with negatively charged BLM can induce some pores. 5. The interaction of drug with s-BLM . Amphotericin B (AmB) is a popular drug frequently applied in the treatment of systemic fungal infections. In the presence of ruthenium (II) as the marker ion, the behavior of AmB to form ion channels in sterol-free and cholesterol- or ergosterol-containing supported phosphatidylcholine bilayer model membranes were studied by CV, a.c. impedance spectroscopy, and UV/visible absorbance spectroscopy. In a fixed cholesterol or ergosterol content (5 mol%) in glassy carbon electrode-supported model membranes, experimental results show that, no matter what form of AmB, monomeric or aggregated, it could form ion channels in supported ergosterol-containing phosphatidylcholine bilayer model membranes. However, AmB could not form ion channels in its monomeric form in sterol-free and cholesterol-containing supported model membranes. When AmB is present as an aggregated state, it can form ion channels in cholesterol-containing supported model membranes. So the state of AmB played an important role in forming ion channels in sterol-free and cholesterol-containing supported phosphatidylcholine bilayer model membranes. 6. The biosensor based on BLM . BLM was constructed on the surface of a glassy carbon electrode. Horseradish peroxidase was embedded into the s-BLM to develop a kind of mediator-free biosensor for H 2 O 2 . The biosensor exhibited fine electrochemical response, stability, and reproducibility due to the presence of the s-BLM. As a model of biological membrane, s-BLM could supply a biological environment for enzyme maintaining its activity. A novel type of potassium sensor based on the capacitance change of valinomycin-incorporated bilayer supported on a gold electrode has been developed and characterized. Investigating the capacitance change allows a simple and specific technique for the measurement of potassium ion in solution. Especially, the homemade capacitance meter is used to monitor the bilayer membrane formation and detect K + . 7. Constructing a hybrid bilayer membrane based on carbon substrate . Carbon material is inert and has a wide potential window in electrochemistry, so modification of carbon materials is of interest to material science and electrochemistry. Primary alkylamine was chemically modified onto the surface of carbon electrode by electrochemical scans, and thus a monolayer was formed on the electrode. Because alkane chains section is towards the outside, a hydrophobic surface is constructed. Then a self-assembled phospholipid monolayer was formed onto the hydrophobic surface. Thus, a new kind of hybrid bilayer membrane (HBM) system was constructed. The formed HBM was characterized by electrochemical and ATR–FTIR methods. From ATR–FTIR results, the lipid order parameter ( S ) of 0.73 was obtained. This kind of hybrid membrane has the advantages of lipid/alkanethiol HBM, such as stability, electrochemical detectability, etc." @default.
- W1488922054 created "2016-06-24" @default.
- W1488922054 creator A5013799893 @default.
- W1488922054 creator A5064857426 @default.
- W1488922054 date "2005-01-01" @default.
- W1488922054 modified "2023-09-23" @default.
- W1488922054 title "Electrochemical Study of the Bilayer Lipid Membrane" @default.
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