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- W1488942802 abstract "Late blight, caused by the oomycete pathogen, Phytophthora infestans, is a devastating disease of potatoes and tomatoes. A key long-term management strategy for combating this disease is to develop potato cultivars with high levels of durable resistance through identification and integration of major resistance genes. The RB gene, cloned from the Mexican diploid potato species Solanum bulbocastanum, confers broad-spectrum resistance to potato late blight. Here, we have determined the late blight resistance phenotypes of eight accessions of Solanum verrucosum, another wild diploid potato species, using greenhouse inoculations and discovered variability among the accessions. While most accessions were resistant, one accession was notably more susceptible. Transcribed orthologs of the RB gene from the eight S. verrucosum accessions were cloned using a homology-based PCR approach. Sequence analysis revealed that the RBver orthologs share up to 83.5% nucleotide identity with RBblb. Stable introduction of the RB ortholog from late blight resistant S. verrucosum PI275260 into susceptible S. tuberosum confers resistance to P. infestans. Interestingly, this functional RBver ortholog contains an insertion of a complete leucine rich repeat when compared to RBblb, and differs from the RBver ortholog from a susceptible accession at only four amino acid residues." @default.
- W1488942802 created "2016-06-24" @default.
- W1488942802 creator A5038597838 @default.
- W1488942802 creator A5060772078 @default.
- W1488942802 date "2006-10-01" @default.
- W1488942802 modified "2023-09-23" @default.
- W1488942802 title "Identification and characterization of RB-orthologous genes from the late blight resistant wild potato species Solanum verrucosum" @default.
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- W1488942802 doi "https://doi.org/10.1016/j.pmpp.2007.05.002" @default.
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