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- W1488971178 abstract "Factors influencing the transformation efficiency of Agrobacterium tumefaciens mediated transformation of Trichoderma harzianum were studied.Through the study,a high efficient transformation system was established.Co-cultivation of T.harzianum Th-33 conidia with A.tumefaciens EHA105 in the presence of acetosyringone resulted in the formation of hygromycin B resistant fungal colonies with high transformation efficient(about 60-150 transformants per 10 conidia).T-DNA insertional library of T. harzianum Th-33 mediated by A.tumefaciens was constructed,which was consisted of more than 8000 transformants.We determined the antagonistic ability of 1260 transformants by antagonistic experiments against Rhizoctonia solani on PDA plate.Twenty-three mutants whose antagonistic ability changed were selected. Five randomly selected antagonistic mutants were proved to be mitotic cell division stable through five generations on PDA plate without hygromycin B.PCR analysis showed that T-DNA was inserted into all transformants genome.These suggest that A.tumefaciens mediated transformation is a potentially powerful tool towards tagged mutagenesis and functional gene clone for T.harzianum." @default.
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- W1488971178 date "2009-01-01" @default.
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- W1488971178 title "The optimization of Agrobacterium tumefaciens mediated Trichoderma harzianum transformation system and chariaterization analysis of antagonistic mutants." @default.
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