Matches in SemOpenAlex for { <https://semopenalex.org/work/W1488989852> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W1488989852 endingPage "4507" @default.
- W1488989852 startingPage "4505" @default.
- W1488989852 abstract "Yeast 5.8 S rRNA is released from purified 26 S rRNA when it is dissolved in water or low salt buffer (50 mM KCl, 10mM Tris-HCl, pH 7.5); it is not released from 60 S ribosomal subunits under similar conditions. The 5.8 S RNA component together with 5 S rRNA can be released from subunits or whole ribosomes by brief heat treatment or in 50% formamide; the Tm for the heat dissociation of 5.8 S RNA is 47 degrees C. This Tm is only slightly lower when 5 S rRNA is released first with EDTA treatment prior to heat treatment. No ribosomal proteins are released by the brief heat treatment. A significant portion of the 5.8 S RNA reassociates with the 60 S subunit when suspended in a higher salt buffer (e.g.0.4 m KCl, 25 mM Tris-HCl, pH 7.5, 6 mM magnesium acetate, 5 mM beta-mercaptoethanol). The Tm of this reassociated complex is also 47 degrees C. The results indicate that in yeast ribosomes the 5.8 S-26 S rRNA interaction is stabilized by ribosomal proteins but that the association is sufficiently loose to permit a reversible dissociation of the 5.8 S rRNA molecule." @default.
- W1488989852 created "2016-06-24" @default.
- W1488989852 creator A5076731737 @default.
- W1488989852 date "1978-07-01" @default.
- W1488989852 modified "2023-09-30" @default.
- W1488989852 title "The release and reassociation of 5.8 S rRNA with yeast ribosomes" @default.
- W1488989852 cites W1424778949 @default.
- W1488989852 cites W1489561579 @default.
- W1488989852 cites W1560163967 @default.
- W1488989852 cites W1683820465 @default.
- W1488989852 cites W1966915929 @default.
- W1488989852 cites W1975263517 @default.
- W1488989852 cites W1985120622 @default.
- W1488989852 cites W1992676142 @default.
- W1488989852 cites W1992878597 @default.
- W1488989852 cites W2017312207 @default.
- W1488989852 cites W2024499727 @default.
- W1488989852 cites W2046293760 @default.
- W1488989852 cites W2080452948 @default.
- W1488989852 cites W2089336233 @default.
- W1488989852 cites W2094224085 @default.
- W1488989852 cites W2100221759 @default.
- W1488989852 cites W44440648 @default.
- W1488989852 doi "https://doi.org/10.1016/s0021-9258(17)30413-1" @default.
- W1488989852 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/350876" @default.
- W1488989852 hasPublicationYear "1978" @default.
- W1488989852 type Work @default.
- W1488989852 sameAs 1488989852 @default.
- W1488989852 citedByCount "16" @default.
- W1488989852 countsByYear W14889898522016 @default.
- W1488989852 crossrefType "journal-article" @default.
- W1488989852 hasAuthorship W1488989852A5076731737 @default.
- W1488989852 hasBestOaLocation W14889898521 @default.
- W1488989852 hasConcept C104317684 @default.
- W1488989852 hasConcept C185592680 @default.
- W1488989852 hasConcept C2779222958 @default.
- W1488989852 hasConcept C55493867 @default.
- W1488989852 hasConcept C67705224 @default.
- W1488989852 hasConcept C67905577 @default.
- W1488989852 hasConcept C86803240 @default.
- W1488989852 hasConcept C88478588 @default.
- W1488989852 hasConceptScore W1488989852C104317684 @default.
- W1488989852 hasConceptScore W1488989852C185592680 @default.
- W1488989852 hasConceptScore W1488989852C2779222958 @default.
- W1488989852 hasConceptScore W1488989852C55493867 @default.
- W1488989852 hasConceptScore W1488989852C67705224 @default.
- W1488989852 hasConceptScore W1488989852C67905577 @default.
- W1488989852 hasConceptScore W1488989852C86803240 @default.
- W1488989852 hasConceptScore W1488989852C88478588 @default.
- W1488989852 hasIssue "13" @default.
- W1488989852 hasLocation W14889898521 @default.
- W1488989852 hasOpenAccess W1488989852 @default.
- W1488989852 hasPrimaryLocation W14889898521 @default.
- W1488989852 hasRelatedWork W1215896252 @default.
- W1488989852 hasRelatedWork W1572513090 @default.
- W1488989852 hasRelatedWork W1995458469 @default.
- W1488989852 hasRelatedWork W1997331300 @default.
- W1488989852 hasRelatedWork W2008993245 @default.
- W1488989852 hasRelatedWork W2028875133 @default.
- W1488989852 hasRelatedWork W2331982037 @default.
- W1488989852 hasRelatedWork W2416109724 @default.
- W1488989852 hasRelatedWork W2917539084 @default.
- W1488989852 hasRelatedWork W3205186342 @default.
- W1488989852 hasVolume "253" @default.
- W1488989852 isParatext "false" @default.
- W1488989852 isRetracted "false" @default.
- W1488989852 magId "1488989852" @default.
- W1488989852 workType "article" @default.