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- W1489006811 abstract "Enzyme activities of V8 protease on AFM tip were investigated by measuring its force curves. The enzyme was immobilized onto the surface of the chemically modified AFM tip; two kinds of peptides were immobilized to the mica surface by a hetero‐reactive reagent. One peptide was designed to be recognized and digested by the enzyme. Another was designed so as not to be recognized by the enzyme. Force curves were obtained by plotting the applied force on the cantilever as a function of the sample displacement. Force applied on the cantilever, over 1.5 nN, was often observed when the tip contacted to a peptide that was digestible by the enzyme. In contrast a force of less than 0.4 nN was observed for contact with other peptides. Large force over 1.5 nN seemed to be caused by rupturing of the intermediate form of the enzyme and the peptide. It was suggested that enzyme molecules on the AFM tip can recognize the target sequence of the peptide. This method can be a tool to analyze biomolecules on a surface." @default.
- W1489006811 created "2016-06-24" @default.
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- W1489006811 date "2003-01-01" @default.
- W1489006811 modified "2023-09-27" @default.
- W1489006811 title "Investigation of Enzyme Activities of the Enzyme Immobilized on an AFM Tip" @default.
- W1489006811 doi "https://doi.org/10.1063/1.1639725" @default.
- W1489006811 hasPublicationYear "2003" @default.
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