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• W1489025024 abstract "Abstract : To study circulating prostate cancer cell adhesion behavior and the contribution to distant metastasis of prostate cancer, we applied a dynamic flow-based E-selectin+SDF-1 coated micro-channel model to isolate and characterize a subpopulation of adhering prostate cancer cells. We found a positive correlation of cells aggressiveness with their adhesion/rolling ability. By using this device we then sorted prostate cancer cell lines (PC3 and DU-145) into rolling and floating populations, and tested for their behavior in vitro and in vivo. Results from our in vitro studies found that rolling cancer cells are more aggressive in nature with higher growth rate and invasion and form bigger and more colonies in soft agar and 3D sphere formation assays. In our in vivo study of orthotopic implantation mouse model, we found that sorted rolling cells are more potent in cases of tumor formation and distance metastasis. In summary, this flow based device provides a platform for isolating the most dangerous, aggressive form of cancer cells which can be used for isolating and characterizing human patients peripheral blood and to form a process for customized cancer treatment." @default.
• W1489025024 created "2016-06-24" @default.
• W1489025024 creator A5078050353 @default.
• W1489025024 date "2013-06-01" @default.
• W1489025024 modified "2023-09-28" @default.
• W1489025024 title "Microvascular Channel Device to Study Aggressiveness in Prostate Cancer Metastasis" @default.
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• W1489025024 doi "https://doi.org/10.21236/ada581896" @default.
• W1489025024 hasPublicationYear "2013" @default.
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