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• W1489065511 endingPage "1829" @default.
• W1489065511 startingPage "1822" @default.
• W1489065511 abstract "Hepatitis A virus (HAV) RNA was extracted from cell culture, serum, liver, and feces and then detected by molecular hybridization with cloned HAV cDNA. Hybridization was approximately 10-fold more sensitive than immune electron microscopy or radioimmunoassay was and less sensitive than was assays of HAV infectivity in primates or in cell culture. As little as 10(3) 50% infective doses of HAV, or approximately 0.1 pg of viral RNA, was detected by this method. Analysis of fecal specimens from an experimentally infected marmoset and an epidemic of hepatitis A showed that HAV excretion could often be detected later in the illness by hybridization than by radioimmunoassay. This technique should be widely applicable for detection and analysis of HAV RNA." @default.
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• W1489065511 date "1987-10-01" @default.
• W1489065511 modified "2023-09-23" @default.
• W1489065511 title "Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization" @default.
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• W1489065511 doi "https://doi.org/10.1128/jcm.25.10.1822-1829.1987" @default.
• W1489065511 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/269349" @default.
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