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- W1489150641 abstract "A technique for biological verification of aflatoxin B 1 was developed based on toxin-mediated induction of lysis in a lysogenic strain of Bacillus megaterium NNRL B-3695. Reduction of culture turbidity was determined at various concentrations of toxin. Incubation of 1.1 × 10 −4 g (dry weight) of cells/ml of growth medium containing 25 μg of B 1 per ml at 37 C reduced initial turbidity 0.20 absorbance units in 4 hr. If the bacterial lysate of the lysogenic strain, after a 2-hr incubation with 25 μg of B 1 per ml, was plated with a sensitive B. megaterium strain (NRRL B-3694), plaque-forming units increased approximately 150 times relative to the control. Comparable testing of the effects of aflatoxin on the nonlysogenic, sensitive strain demonstrated that 75 μg of B 1 per ml neither induced lysis nor plaque-forming units. Although induction is not an exclusive property of aflatoxin B 1 , the differential response of the lysogenic and sensitive Bacillus strains to B 1 offers a unique and rapid technique for biological verification of the toxin." @default.
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- W1489150641 date "1970-11-01" @default.
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- W1489150641 title "Aflatoxin B 1 Induction of Lysogenic Bacteria" @default.
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- W1489150641 doi "https://doi.org/10.1128/am.20.5.782-785.1970" @default.
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