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• W1489183905 startingPage "8913" @default.
• W1489183905 abstract "The purification and characterization of transcription factor IIF (TFIIF), a factor required for transcription by the RNA polymerase II machinery, is described. TFIIF was isolated from the previously described IIE protein fraction. TFIIF enters into the transcription cycle via a preinitiation complex, and it is required for the formation of a complex capable of initiating transcription in the presence of heparin concentrations that inhibit the action of a free factor. TFIIF and TFIIE independently interacted with purified RNA polymerase II. TFIIF and TFIIE were both required for transcription of several class II promoters, including a promoter that lacks the conserved TATA box. Interestingly TFIIF was absolutely required for the formation of a preinitiation complex; however, it also affected the elongation phase of the transcription cycle. TFIIF, together with the previously described elongation factor TFIIS, was required for efficient elongation." @default.
• W1489183905 created "2016-06-24" @default.
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• W1489183905 date "1989-05-01" @default.
• W1489183905 modified "2023-09-30" @default.
• W1489183905 title "Factors Involved in Specific Transcription by Mammalian RNA Polymerase II" @default.
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• W1489183905 doi "https://doi.org/10.1016/s0021-9258(18)81880-4" @default.
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