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- W1489223613 abstract "Fertilized eggs of Ilyanassa obsoleta form a protuberance which resembles a normal polar lobe when injected with Sr2+ or Ca2+ by microiontophoresis. Eggs also form a lobe-like protuberance when exposed to any of three drugs: compound 4880, ionophore X537A, and ionophore A23187. Protuberances form more quickly and at lower drug concentrations if additional exogenous Ca2+ is added, whereas higher concentrations of Mg2+ do not have such an effect. When eggs are exposed to these drugs in Ca2+-, Mg2+-“free” seawater, with or without 10 mM EDTA, the eggs are still able to undergo extensive shape changes and form protuberances. Drug-induced shape changes are prevented by cytochalasin B, but will still occur in the presence of colchicine. Approximately 75% of Ilyanassa eggs are capable of forming and resorbing their third polar lobe and undergoing cytokinesis in Ca2+-, Mg2+-“free” artificial seawater (even containing 10 mM EDTA), solutions which by atomic absorption spectroscopy are shown to contain low concentrations of Ca2+ (3–5 μM) and Mg2+ (1.0–3.5 μM). The data suggest that if Ca2+ is required for normal polar lobe formation and cytokinesis, it is derived from intracellular sources or is required in only very low exogenous concentrations (i.e., less than 10−2 μM free Ca2+, in the presence of 10 mM EDTA)." @default.
- W1489223613 created "2016-06-24" @default.
- W1489223613 creator A5085735959 @default.
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- W1489223613 date "1980-01-01" @default.
- W1489223613 modified "2023-10-17" @default.
- W1489223613 title "Polar lobe formation and cytokinesis in fertilized eggs of Ilyanassa obsoleta" @default.
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- W1489223613 doi "https://doi.org/10.1016/0012-1606(80)90058-5" @default.
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