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• W1489284738 endingPage "1187" @default.
• W1489284738 startingPage "1178" @default.
• W1489284738 abstract "Experiments employing electron transport inhibitors, room- and low-temperature spectroscopy, and photochemical action spectra have led to a model for the respiratory chain of Pseudomonas carboxydovorans. The chain is branched at the level of b-type cytochromes or ubiquinone. One branch (heterotrophic branch) contained cytochromes b558, c, and a1; the second branch (autotrophic branch) allowed growth in the presence of CO and contained cytochromes b561 and o (b563). Electrons from the oxidation of organic substrates were predominantly channelled into the heterotrophic branch, whereas electrons derived from the oxidation of CO or H2 could use both branches. Tetramethyl-p-phenylenediamine was oxidized via cytochromes c and a exclusively. The heterotrophic branch was sensitive to antimycin A, CO, and micromolar concentrations of cyanide. The autotrophic branch was sensitive to 2-n-heptyl-4-hydroxyquinoline-N-oxide, insensitive to CO, and inhibited only by millimolar concentrations of cyanide. The functioning of cytochrome a1 as a terminal oxidase was established by photochemical action spectra. Reoxidation experiments established the functioning of cytochrome o as an alternative CO-insensitive terminal oxidase of the autotrophic branch." @default.
• W1489284738 created "2016-06-24" @default.
• W1489284738 creator A5027041123 @default.
• W1489284738 creator A5056310735 @default.
• W1489284738 date "1983-12-01" @default.
• W1489284738 modified "2023-10-03" @default.
• W1489284738 title "Carbon monoxide-insensitive respiratory chain of Pseudomonas carboxydovorans" @default.
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• W1489284738 doi "https://doi.org/10.1128/jb.156.3.1178-1187.1983" @default.
• W1489284738 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/217965" @default.
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