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- W1489285616 abstract "To better understand how the composition of bacterial communities changes in response to different environmental conditions, we examined the influence of increasing phenol load on the distribution of the protein-coding functional gene of the largest subunit of phenol hydroxylase (LmPH) and of the 16S rRNA gene in lab-scale activated sludge reactors. LmPH diversity was assessed initially from a total of 124 clone sequences retrieved from two reactors exposed to a low (0.25 g L−1) and a high (2.5 g L−1) phenol concentration. The quantitative changes in the concentration of the eight detected genotypes accompanied changes in the phenol degradation rates, indicating a community structure–function relationship. Nonmetric dimensional analysis showed that LmPH genotypes and the denaturing gradient gel electrophoresis banding patterns clustered together by phenol concentration, rather than by reactor identity. Seven isolates, representing cultivated strains of each of the observed LmPH genotypes, exhibited a rather narrow range of physiological diversity, in terms of the growth rate and the kinetic parameters of the phenol-degrading activity. We suggest that lab-scale reactors support many ecological niches, which allow the maintenance of a high diversity of ecotypes through varying concentrations of phenol, but the ability of particular strains to become dominant members of the community under the different environmental conditions cannot be predicted easily solely from their phenol-degrading properties." @default.
- W1489285616 created "2016-06-24" @default.
- W1489285616 creator A5011863548 @default.
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- W1489285616 date "2010-05-04" @default.
- W1489285616 modified "2023-10-07" @default.
- W1489285616 title "Maintenance of phenol hydroxylase genotypes at high diversity in bioreactors exposed to step increases in phenol loading" @default.
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- W1489285616 doi "https://doi.org/10.1111/j.1574-6941.2010.00898.x" @default.
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