Matches in SemOpenAlex for { <https://semopenalex.org/work/W1489292103> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1489292103 endingPage "8614" @default.
- W1489292103 startingPage "8609" @default.
- W1489292103 abstract "An enzyme that hydrolyzes the O-glycosidic linkage between alpha-N-acetyl-D-galactosamine and serine or threonine in mucins and mucin-type glycoproteins was purified by chromatography on an Affi-Gel 202 column or isoelectric focusing from filtrates of Diplococcus pneumoniae cultures. The final preparations were free of protease and a wide range of other glycosidase activities. The preparation obtained by isoelectric focusing was shown to consist of a single protein by gel filtration and sodium dodecyl sulfate-gel electrophoresis. This preparation had an apparent molecular weight of about 160,000, determined by gel filtration, an optimum pH of 7.6, and an isoelectric point in the range pH 8 to 9. The enzyme releases the disaccharide Gal-GalNAc from a variety of glycopeptide and glycoprotein substrates and appears to have a specific requirement for an unsubstituted galactose in the nonreducing terminus and an alpha linkage between N-acetylgalactosamine and the aglycone. This is the only endoenzyme known capable of cleaving the linkage between a carbohydrate and serine or threonine residues in glycoproteins. The ability of this enzyme to act on macromolecular substrates and its pH optimum makes it ideally suited to explore the distribution and function of mucin-type glycoproteins on normal and cancer cell surfaces." @default.
- W1489292103 created "2016-06-24" @default.
- W1489292103 creator A5039204162 @default.
- W1489292103 creator A5071189035 @default.
- W1489292103 creator A5085129708 @default.
- W1489292103 date "1977-12-01" @default.
- W1489292103 modified "2023-10-14" @default.
- W1489292103 title "Purification and properties of an endo-alpha-N-acetyl-D-galactosaminidase from Diplococcus pneumoniae" @default.
- W1489292103 cites W1481763897 @default.
- W1489292103 cites W1486423971 @default.
- W1489292103 cites W1535002104 @default.
- W1489292103 cites W1537777030 @default.
- W1489292103 cites W1551383822 @default.
- W1489292103 cites W1558637401 @default.
- W1489292103 cites W1564905326 @default.
- W1489292103 cites W1750773183 @default.
- W1489292103 cites W1897055678 @default.
- W1489292103 cites W1949848638 @default.
- W1489292103 cites W1974457112 @default.
- W1489292103 cites W1978247002 @default.
- W1489292103 cites W1994606229 @default.
- W1489292103 cites W2048705400 @default.
- W1489292103 cites W2071893027 @default.
- W1489292103 cites W2075996902 @default.
- W1489292103 cites W2080676657 @default.
- W1489292103 cites W2080750481 @default.
- W1489292103 cites W2092550409 @default.
- W1489292103 cites W2095114866 @default.
- W1489292103 cites W2100837269 @default.
- W1489292103 cites W2321887298 @default.
- W1489292103 cites W2403657425 @default.
- W1489292103 doi "https://doi.org/10.1016/s0021-9258(19)75264-8" @default.
- W1489292103 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21877" @default.
- W1489292103 hasPublicationYear "1977" @default.
- W1489292103 type Work @default.
- W1489292103 sameAs 1489292103 @default.
- W1489292103 citedByCount "228" @default.
- W1489292103 countsByYear W14892921032012 @default.
- W1489292103 countsByYear W14892921032013 @default.
- W1489292103 countsByYear W14892921032014 @default.
- W1489292103 countsByYear W14892921032015 @default.
- W1489292103 countsByYear W14892921032018 @default.
- W1489292103 countsByYear W14892921032020 @default.
- W1489292103 crossrefType "journal-article" @default.
- W1489292103 hasAuthorship W1489292103A5039204162 @default.
- W1489292103 hasAuthorship W1489292103A5071189035 @default.
- W1489292103 hasAuthorship W1489292103A5085129708 @default.
- W1489292103 hasBestOaLocation W14892921031 @default.
- W1489292103 hasConcept C159110408 @default.
- W1489292103 hasConcept C185592680 @default.
- W1489292103 hasConcept C2775944032 @default.
- W1489292103 hasConcept C2781253189 @default.
- W1489292103 hasConcept C49453240 @default.
- W1489292103 hasConcept C501593827 @default.
- W1489292103 hasConcept C55493867 @default.
- W1489292103 hasConcept C64943373 @default.
- W1489292103 hasConcept C71924100 @default.
- W1489292103 hasConcept C86803240 @default.
- W1489292103 hasConcept C89423630 @default.
- W1489292103 hasConceptScore W1489292103C159110408 @default.
- W1489292103 hasConceptScore W1489292103C185592680 @default.
- W1489292103 hasConceptScore W1489292103C2775944032 @default.
- W1489292103 hasConceptScore W1489292103C2781253189 @default.
- W1489292103 hasConceptScore W1489292103C49453240 @default.
- W1489292103 hasConceptScore W1489292103C501593827 @default.
- W1489292103 hasConceptScore W1489292103C55493867 @default.
- W1489292103 hasConceptScore W1489292103C64943373 @default.
- W1489292103 hasConceptScore W1489292103C71924100 @default.
- W1489292103 hasConceptScore W1489292103C86803240 @default.
- W1489292103 hasConceptScore W1489292103C89423630 @default.
- W1489292103 hasIssue "23" @default.
- W1489292103 hasLocation W14892921031 @default.
- W1489292103 hasOpenAccess W1489292103 @default.
- W1489292103 hasPrimaryLocation W14892921031 @default.
- W1489292103 hasRelatedWork W1976925323 @default.
- W1489292103 hasRelatedWork W2017369909 @default.
- W1489292103 hasRelatedWork W2026514246 @default.
- W1489292103 hasRelatedWork W2048846203 @default.
- W1489292103 hasRelatedWork W2134440230 @default.
- W1489292103 hasRelatedWork W2148349589 @default.
- W1489292103 hasRelatedWork W2382739716 @default.
- W1489292103 hasRelatedWork W2433393987 @default.
- W1489292103 hasRelatedWork W2606245082 @default.
- W1489292103 hasRelatedWork W4221125579 @default.
- W1489292103 hasVolume "252" @default.
- W1489292103 isParatext "false" @default.
- W1489292103 isRetracted "false" @default.
- W1489292103 magId "1489292103" @default.
- W1489292103 workType "article" @default.