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- W1489356438 abstract "amelogenin, or ZFX/ZFY. During the sex determination using the SRY region, male samples yield a product, whereas female samples do not. Therefore, this system requires two primer sets, one for the SRY region and another as an internal positive control. On the other hand, the amelogenin and the zinc finger (ZFX/ZFY) regions require only one primer set to amplify a region of the X–Y homologous gene. In the ZFX/ZFY system, sex is determined by the appearance of sex-specific bands in restriction fragment length polymorphism (RFLP) assays after PCR. Aasen and Medrano (1990) assayed ZFX/ZFY for humans, cattle, sheep, and goats using universal primers. They amplified 447 or 445 bp fragments from male or female genomic DNA corresponding to the ZFY or ZFX genes. They could not find RFLPs in horse fragments with the group of enzymes chosen for the other species. However, Senese et al. (1999) reported a" @default.
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- W1489356438 date "2006-01-01" @default.
- W1489356438 modified "2023-09-22" @default.
- W1489356438 title "SEX VERIFICATION OF ARAB HORSES USING PCR-RFLP WITHIN ZFX/ZFY GENE" @default.
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