FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1489366295> ?p ?o ?g. }

• W1489366295 endingPage "174" @default.
• W1489366295 startingPage "174" @default.
• W1489366295 abstract "Vitrification has become the preferred method for cryopreserving in vitro-produced bovine embryos (IVP). Here we introduce a technique for vitrification developed at CryoLogic (the CLV Method), in conjunction with a study comparing the post-thaw viability of IVP embryos frozen by the widely used open pulled straw method (OPS—Vajta et al., 1997 Cryo-Letters 18, 191) and the new CLV Method. Vitrification on thin metal surfaces has been explored and demonstrated previously (Le Gal &amp; Massip 1999, Cryobiology 38, 290), and Dinnyes presented a Solid Surface Vitrification (SSV) (Dinnyes et al., 2000, Biol. Reprod. 63, 513). The CLV Method utilizes vitrification on the surface of a solid metal block. This surface has been custom shaped and treated to enhance vitreous formation. The method also includes handling, storage and thawing protocols designed to avoid damage from crystallization of the unstable glass. Briefly, the block is precooled in LN2 to −196°C. Up to 10 embryos are collected into a droplet of medium (3μL), on the end of a fibre carrier attached to a handle. The droplet is presented to the vitrification surface, where it is converted into a glass bead by cooling rates comparable to that of plunging into solid/liquid phase nitrogen (−210°C) (Arav et al., 2001 Theriogenology 55, 313). The glass bead, fibre and handle are transferred quickly into a half-sealed, precooled straw, and the handle seals the open end. A bead is thawed very rapidly by removing the handle, fibre and bead from the straw and transferring the bead into washing medium. COCs collected from bovine ovaries obtained from abattoirs were matured, fertilized and cultured for 6 days (Fry et al., 2003 Theriogenology 59, 446). Embryos reaching the blastocyst or expanding blastocyst stage of development were graded (Grades 1, 2, and 3), equilibrated for 5min in HEPES-199 medium with 20% FCS (HFm), placed in HFm with 10% EG, and 10% DMSO (VS1) for 2min, and then transferred to HFm with 20% EG, 20% DMSO (VS2) for 30s (Vajta). Between 5 and 10 IVP embryos were processed and collected for vitrification, either in an OPS plunged into LN2, or in a 3μL droplet vitrified by the CLV Method. The two sets of specimens were stored in LN2, and later thawed. Both OPS tips and beads were thawed in 0.5mL of HFm with 0.2M sucrose at 39°C. Embryos were maintained at 39°C, examined after 5min for contraction, and again after 6 h for re-expansion. They were then transferred to culture medium, incubated and examined at 24 and 48h to assess hatching. As shown in Table 1, the CLV method appears to be satisfactory for maintaining membrane integrity (expansion) and developmental potential (hatching) for even poorer grade embryos, that might be more sensitive to the stresses of cryopreservation. Table 1 Re-expansion and hatching rates of graded thawed bovine embryos vitrified by OPS or CLV methods" @default.
• W1489366295 created "2016-06-24" @default.
• W1489366295 creator A5009813274 @default.
• W1489366295 creator A5011141000 @default.
• W1489366295 creator A5022107967 @default.
• W1489366295 creator A5024478320 @default.
• W1489366295 creator A5090078212 @default.
• W1489366295 date "2004-01-01" @default.
• W1489366295 modified "2023-09-27" @default.
• W1489366295 title "105VITRIFICATION OF BOVINE EMBRYOS USING THE CLV METHOD" @default.
• W1489366295 doi "https://doi.org/10.1071/rdv16n1ab105" @default.
• W1489366295 hasPublicationYear "2004" @default.
• W1489366295 type Work @default.
• W1489366295 sameAs 1489366295 @default.
• W1489366295 citedByCount "11" @default.
• W1489366295 countsByYear W14893662952012 @default.
• W1489366295 countsByYear W14893662952013 @default.
• W1489366295 countsByYear W14893662952014 @default.
• W1489366295 countsByYear W14893662952018 @default.
• W1489366295 countsByYear W14893662952021 @default.
• W1489366295 crossrefType "journal-article" @default.
• W1489366295 hasAuthorship W1489366295A5009813274 @default.
• W1489366295 hasAuthorship W1489366295A5011141000 @default.
• W1489366295 hasAuthorship W1489366295A5022107967 @default.
• W1489366295 hasAuthorship W1489366295A5024478320 @default.
• W1489366295 hasAuthorship W1489366295A5090078212 @default.
• W1489366295 hasBestOaLocation W14893662951 @default.
• W1489366295 hasConcept C109386097 @default.
• W1489366295 hasConcept C159985019 @default.
• W1489366295 hasConcept C16685009 @default.
• W1489366295 hasConcept C179104552 @default.
• W1489366295 hasConcept C185592680 @default.
• W1489366295 hasConcept C192562407 @default.
• W1489366295 hasConcept C2778022349 @default.
• W1489366295 hasConcept C2779587293 @default.
• W1489366295 hasConcept C71924100 @default.
• W1489366295 hasConceptScore W1489366295C109386097 @default.
• W1489366295 hasConceptScore W1489366295C159985019 @default.
• W1489366295 hasConceptScore W1489366295C16685009 @default.
• W1489366295 hasConceptScore W1489366295C179104552 @default.
• W1489366295 hasConceptScore W1489366295C185592680 @default.
• W1489366295 hasConceptScore W1489366295C192562407 @default.
• W1489366295 hasConceptScore W1489366295C2778022349 @default.
• W1489366295 hasConceptScore W1489366295C2779587293 @default.
• W1489366295 hasConceptScore W1489366295C71924100 @default.
• W1489366295 hasIssue "2" @default.
• W1489366295 hasLocation W14893662951 @default.
• W1489366295 hasOpenAccess W1489366295 @default.
• W1489366295 hasPrimaryLocation W14893662951 @default.
• W1489366295 hasRelatedWork W1985245687 @default.
• W1489366295 hasRelatedWork W2018720806 @default.
• W1489366295 hasRelatedWork W2111545704 @default.
• W1489366295 hasRelatedWork W2359153294 @default.
• W1489366295 hasRelatedWork W2368622673 @default.
• W1489366295 hasRelatedWork W2396636734 @default.
• W1489366295 hasRelatedWork W2771804529 @default.
• W1489366295 hasRelatedWork W2899084033 @default.
• W1489366295 hasRelatedWork W2981922900 @default.
• W1489366295 hasRelatedWork W3082375464 @default.
• W1489366295 hasVolume "16" @default.
• W1489366295 isParatext "false" @default.
• W1489366295 isRetracted "false" @default.
• W1489366295 magId "1489366295" @default.
• W1489366295 workType "article" @default.