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- W1489422762 abstract "1. N-(p-(2-Benzimidazolyl)phenyl)maleimide (BIPM) has been used for the quantitative determination of thiol compounds. The method is based on the particular fluorescence characteristics of this reagent: its adducts to thiol compounds have strong fluorescence (λmax, ex 315 mμ, λmax, em 365 mμ), while the reagent itself has no appreciable fluorescence. 2. The addition reaction of 2 × 10−6M BIPM to thiols is completed within 30 min at pH 6.85 and 0°C. A linear relation was observed up to 10−5M of the reagent between concentration of adduct and intensity of fluorescence. 3. A very sensitive fluorometric determination of thiol compounds has been developed based on the above results. The detection limit was 10−6M concentration of thiols, with 0.1 ml of sample, corresponding to 0.03 μg reduced glutathione, with an accuracy of 3%. 4. Using the fluorometric method, the oxidation rate of glutathione catalyzed by metal ions was examined and the contents of thiols in human lacrimal fluid and seurm were determined." @default.
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- W1489422762 date "1972-08-01" @default.
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- W1489422762 title "Fluorescent thiol reagents" @default.
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- W1489422762 doi "https://doi.org/10.1016/0003-2697(72)90111-x" @default.
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