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- W1489498997 abstract "We have mapped three promoters on a plasmid that contains the rrnB operon of Escherichia coli and the attP′ region of λ (Brosius et al., 1980b). One of these promoters is a previously unmapped promoter, termed λPL57, that is located on the attP′ arm of λ, and from which transcription proceeds through the att core sequence. Sequence analysis of this promoter reveals striking homologies to the presumed CII binding sequences of λPI and λPE (Abraham et al., 1980; Hoess et al., 1980), suggesting that this promoter may be involved in establishment of lysogeny. The other two promoters we have mapped are those of the rrnB operon. We have used electron microscopy to quantitate the binding of E. coli RNA polymerase to these promoters in the presence and absence of the regulatory nucleotide guanosine tetraphosphate. These studies show that guanosine tetraphosphate specifically decreases the ability of RNA polymerase to bind to each of the two rrnB promoters, while not affecting the binding to the λPL57 promoter." @default.
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- W1489498997 date "1981-03-01" @default.
- W1489498997 modified "2023-09-25" @default.
- W1489498997 title "Transcriptional mapping of plasmid pKK3535" @default.
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- W1489498997 doi "https://doi.org/10.1016/0022-2836(81)90041-3" @default.
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