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• W1489505539 endingPage "1819" @default.
• W1489505539 startingPage "1811" @default.
• W1489505539 abstract "Replication of the IncL/M plasmid pMU604 is controlled by a small antisense RNA molecule (RNAI), which, by inhibiting the formation of an RNA pseudoknot, regulates translation of the replication initiator protein, RepA. Efficient translation of the repA mRNA was shown to require the translation and correct termination of the leader peptide, RepB, and the formation of the pseudoknot. Although the pseudoknot was essential for the expression of repA, its presence was shown to interfere with the translation of repB. The requirement for pseudoknot formation could in large part be obviated by improving the ribosome binding region of repA, either by replacing the GUG start codon by AUG or by increasing the spacing between the start codon and the Shine-Dalgarno sequence (SD). The spacing between the distal pseudoknot sequence and the repA SD was shown to be suboptimal for maximal expression of repA." @default.
• W1489505539 created "2016-06-24" @default.
• W1489505539 creator A5043623870 @default.
• W1489505539 creator A5056140747 @default.
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• W1489505539 date "1999-03-15" @default.
• W1489505539 modified "2023-10-15" @default.
• W1489505539 title "Analysis of Elements Involved in Pseudoknot-Dependent Expression and Regulation of the <i>repA</i> Gene of an IncL/M Plasmid" @default.
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• W1489505539 doi "https://doi.org/10.1128/jb.181.6.1811-1819.1999" @default.
• W1489505539 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/93579" @default.
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• W1489505539 hasPublicationYear "1999" @default.
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