Matches in SemOpenAlex for { <https://semopenalex.org/work/W1489553970> ?p ?o ?g. }
- W1489553970 endingPage "1354" @default.
- W1489553970 startingPage "1341" @default.
- W1489553970 abstract "Crohn's disease (CD) and ulcerative colitis (UC) are associated with chronic tissue damage and continuous tissue repair. A central, but not well-characterized, event during this process is the migration of activated fibroblasts to the wound.Human colonic lamina propria fibroblasts (CLPF) were isolated from patients with CD and UC and from healthy controls and were characterized by immunocytochemistry. Migration assays of CLPF were performed in the modified 48-well Boyden chamber. Focal adhesion kinase (FAK) and FAK autophosphorylation in migrating CLPF were determined by Western blotting. FAK mRNA expression was investigated by Northern blotting.The migration of CD-CLPF and UC-CLPF was significantly reduced when compared with control-CLPF. This was correlated with a decrease in FAK phosphorylation, whereas, in migrating control-CLPF, an increase was found. Similarly, the presence of the inflammatory mediators interferon (IFN)-gamma (50 ng/mL) or tumor necrosis factor (TNF) (30 ng/mL) in conditioned medium significantly reduced the migration of control-CLPF to 41% +/- 4% or 30% +/- 7%, respectively. Preincubation of control-CLPF with TNF (20 ng/mL) and IFN-gamma (10 ng/mL) for 3 days reduced their migratory response to 10% of control (P < 0.001), which also was correlated with a decrease in FAK phosphorylation. Culture of IFN-gamma/TNF-treated CLPF for a further 7 days without cytokines did not restore the migratory potential and FAK phosphorylation, indicating a persistent functional change.CD- and UC-CLPF have a reduced migratory potential compared with normal CLPF. That may be caused by contact with IFN-gamma and TNF. This loss of migratory potential was correlated with diminished FAK phosphorylation." @default.
- W1489553970 created "2016-06-24" @default.
- W1489553970 creator A5002148720 @default.
- W1489553970 creator A5013989759 @default.
- W1489553970 creator A5042336970 @default.
- W1489553970 creator A5043330032 @default.
- W1489553970 creator A5051726567 @default.
- W1489553970 creator A5058012401 @default.
- W1489553970 creator A5065334962 @default.
- W1489553970 creator A5085788744 @default.
- W1489553970 creator A5088721301 @default.
- W1489553970 date "2003-11-01" @default.
- W1489553970 modified "2023-10-10" @default.
- W1489553970 title "Reduced migration of fibroblasts in inflammatory bowel disease: role of inflammatory mediators and focal adhesion kinase1 1The authors thank Dr. Luitpold E. Miller for his assistance in the preparation of this manuscript; the doctors and nurses of the Endoscopy department; the surgeons and pathologists of the University of Regensburg, especially Dr. Frauke Bataille, for their ongoing great cooperation; and, most importantly, all patients who agreed to give biopsy and tissue samples, without …" @default.
- W1489553970 cites W1642457845 @default.
- W1489553970 cites W1670573097 @default.
- W1489553970 cites W1929023701 @default.
- W1489553970 cites W1965396386 @default.
- W1489553970 cites W1997474028 @default.
- W1489553970 cites W2000508492 @default.
- W1489553970 cites W2002440725 @default.
- W1489553970 cites W2008192236 @default.
- W1489553970 cites W2014280822 @default.
- W1489553970 cites W2016090787 @default.
- W1489553970 cites W2020824003 @default.
- W1489553970 cites W2023325143 @default.
- W1489553970 cites W2028509749 @default.
- W1489553970 cites W2046371371 @default.
- W1489553970 cites W2057275950 @default.
- W1489553970 cites W2061017342 @default.
- W1489553970 cites W2088041505 @default.
- W1489553970 cites W2090109575 @default.
- W1489553970 cites W2098307303 @default.
- W1489553970 cites W2101612079 @default.
- W1489553970 cites W2108572357 @default.
- W1489553970 cites W2120484559 @default.
- W1489553970 cites W2131790315 @default.
- W1489553970 cites W2133733169 @default.
- W1489553970 cites W2133904881 @default.
- W1489553970 cites W2173180277 @default.
- W1489553970 cites W2413827366 @default.
- W1489553970 cites W4248973968 @default.
- W1489553970 cites W4297812125 @default.
- W1489553970 doi "https://doi.org/10.1016/j.gastro.2003.07.004" @default.
- W1489553970 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14598250" @default.
- W1489553970 hasPublicationYear "2003" @default.
- W1489553970 type Work @default.
- W1489553970 sameAs 1489553970 @default.
- W1489553970 citedByCount "92" @default.
- W1489553970 countsByYear W14895539702012 @default.
- W1489553970 countsByYear W14895539702013 @default.
- W1489553970 countsByYear W14895539702014 @default.
- W1489553970 countsByYear W14895539702015 @default.
- W1489553970 countsByYear W14895539702016 @default.
- W1489553970 countsByYear W14895539702017 @default.
- W1489553970 countsByYear W14895539702018 @default.
- W1489553970 countsByYear W14895539702019 @default.
- W1489553970 countsByYear W14895539702020 @default.
- W1489553970 countsByYear W14895539702021 @default.
- W1489553970 countsByYear W14895539702022 @default.
- W1489553970 countsByYear W14895539702023 @default.
- W1489553970 crossrefType "journal-article" @default.
- W1489553970 hasAuthorship W1489553970A5002148720 @default.
- W1489553970 hasAuthorship W1489553970A5013989759 @default.
- W1489553970 hasAuthorship W1489553970A5042336970 @default.
- W1489553970 hasAuthorship W1489553970A5043330032 @default.
- W1489553970 hasAuthorship W1489553970A5051726567 @default.
- W1489553970 hasAuthorship W1489553970A5058012401 @default.
- W1489553970 hasAuthorship W1489553970A5065334962 @default.
- W1489553970 hasAuthorship W1489553970A5085788744 @default.
- W1489553970 hasAuthorship W1489553970A5088721301 @default.
- W1489553970 hasBestOaLocation W14895539701 @default.
- W1489553970 hasConcept C104317684 @default.
- W1489553970 hasConcept C11960822 @default.
- W1489553970 hasConcept C142724271 @default.
- W1489553970 hasConcept C151166631 @default.
- W1489553970 hasConcept C153911025 @default.
- W1489553970 hasConcept C164027704 @default.
- W1489553970 hasConcept C17991360 @default.
- W1489553970 hasConcept C185592680 @default.
- W1489553970 hasConcept C203014093 @default.
- W1489553970 hasConcept C2776055305 @default.
- W1489553970 hasConcept C2776914184 @default.
- W1489553970 hasConcept C2778260677 @default.
- W1489553970 hasConcept C2778690821 @default.
- W1489553970 hasConcept C2779134260 @default.
- W1489553970 hasConcept C2780269544 @default.
- W1489553970 hasConcept C2780841837 @default.
- W1489553970 hasConcept C502942594 @default.
- W1489553970 hasConcept C55493867 @default.
- W1489553970 hasConcept C71924100 @default.
- W1489553970 hasConcept C85265743 @default.
- W1489553970 hasConcept C86803240 @default.
- W1489553970 hasConceptScore W1489553970C104317684 @default.
- W1489553970 hasConceptScore W1489553970C11960822 @default.
- W1489553970 hasConceptScore W1489553970C142724271 @default.
- W1489553970 hasConceptScore W1489553970C151166631 @default.
- W1489553970 hasConceptScore W1489553970C153911025 @default.