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- W1489735852 abstract "Adherence represents one important and initial virulence factor of fungal pathogenicity. In the model fungus Saccharomyces cerevisiae adherence to substrates or to other cells depends on nutrients and is part of complex developmental processes, such as haploid invasive growth or diploid pseudohyphal formation. Adherence per se can also be induced by amino acid starvation. This specific adaptation requires the adhesin Flo11p and the transcriptional activator of the general amino acid control system Gcn4p. A genome-wide transcriptional analysis of ∑1278b yeast cells under adhesion-inducing conditions imposed by amino acid starvation was performed to identify specifically regulated genes. 22 novel genes were inducible by amino acid starvation. 72 genes of different functional groups showed a previously unrecognized dependence upon Gcn4p under adhesion-inducing conditions. In addition, several genes were identified as inducible by amino acid starvation in a Gcn4p-independent manner. 2D-DIGE experiments of ∑1278b yeast cells were carried out to identify regulated proteins under adhesion-inducing conditions. Seven protein spots displayed a highly increased intensity in response to amino acid starvation. These protein spots were identified by mass spectrometry as Cpc2p, Efb1p, His1p, Hsp60p, Sod1p, Tpi1p and Tpm1p. Comparisons with the respective transcriptional profiles revealed that the mRNA levels of the encoding genes were significantly increased only for the HIS1 gene. Deletion of CPC2, which encodes a highly conserved G?-like WD-repeat protein, results in an adhesion deficient phenotype of amino acid-starved yeast cells. CPC2 is also required for basal expression and activation of FLO11 under amino acid starvation. The adherence-dependent developmental processes of haploid invasive growth and diploid pseudohyphal formation also depend on CPC2. During utilization of the fermentable carbon source glucose, transcription of CPC2 is induced. CPC2 promoter analyses were performed to analyse regulation, and identified two upstream activation sequence elements required for basal expression and regulation of CPC2. The forkhead-like transcription factor Fhl1p and its co-factor Ifh1p were found as trans-acting elements. Deletion of FHL1 reduces CPC2 transcription significantly in presence of glucose, whereas increased amounts of Ifh1p induces CPC2 transcription even under utilization of the non-fermentable carbon source ethanol." @default.
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- W1489735852 date "2005-11-16" @default.
- W1489735852 modified "2023-09-27" @default.
- W1489735852 title "Regulation of gene expression and adhesion in Saccharomyces cerevisiae" @default.
- W1489735852 hasPublicationYear "2005" @default.
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